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SHR Neuro Cancer Cardio Lipid Metab Microb
Cervar-Zivkovic, M; Hu, C; Barton, A; Sadovsky, Y; Desoye, G; Lang, U; Nelson, DM.
Endothelin-1 attenuates apoptosis in cultured trophoblasts from term human placentas.
Reprod Sci. 2007; 14(5):430-439
Doi: 10.1177/1933719107305801
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Cervar-Zivkovic Mila
- Co-authors Med Uni Graz
- Desoye Gernot
- Lang Uwe
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- Abstract:
- The authors test the hypothesis that endothelin-1 (ET-1) modulates apoptosis in human term trophoblasts. Primary cultures of cytotrophoblasts from term human placentas (n = 5) were cultured for 16 hours total or 24 hours prior to harvest at 72 hours in atmospheres of <1%, 8%, and 20% oxygen, in the presence of 10% serum, ET-1 (1-100 pmol/mL), both, or neither. The apoptotic cleavage products of poly-ADP-ribose polymerase and cytokeratin 18 filaments were quantified by Western analysis and immunocytochemistry. The expression of BAD, pBAD-serine 112, p53, and 2 isoforms of MDM2 were quantified by immunoblotting, and endothelin A and B receptors were analyzed by immunocytochemistry. Compared to vehicle control, increasing concentrations of ET-1 reduce by 3- to 6-fold the level of apoptosis in cytotrophoblasts exposed to serum-free conditions at 20% oxygen. Similarly, syncytiotrophoblast cultures grown for 24 hours without serum in 100 pmol/mL ET-1 show a 3-fold lower level of apoptosis compared with vehicle control. ET-1 significantly reduces apoptosis in cultures exposed to 20% oxygen but not in cultures exposed to 8% or 1% oxygen. The effect of ET-1 on apoptosis in 20% oxygen is accompanied by reduced p53 expression and is correlated with enhanced expression of endothelin B receptor, compared to cultures in 8% or 1% oxygen. ET-1 reduces apoptosis in cultured human trophoblasts, and this finding suggests a role for ET-1 in protecting trophoblasts against injury.
- Find related publications in this database (using NLM MeSH Indexing)
- Apoptosis - physiology
- Cells, Cultured -
- Culture Media, Serum-Free -
- Endothelin-1 - physiology
- Female -
- Humans -
- Labor, Obstetric - physiology
- Placenta - cytology Placenta - physiology
- Pregnancy -
- Trophoblasts - cytology Trophoblasts - physiology
- Find related publications in this database (Keywords)
- endothelin-1
- apoptosis
- trophoblast
- placenta