Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Singer, G; Houghton, J; Rivera, CA; Anthoni, C; Granger, DN.
Role of LPS in the hepatic microvascular dysfunction elicited by cecal ligation and puncture in mice.
J Hepatol. 2007; 47(6): 799-806.
Doi: 10.1016/j.jhep.2007.07.021
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Singer Georg
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- BACKGROUND/AIMS: Sepsis remains a leading cause of death in critically ill patients. Because endotoxemia is viewed as a key mediator of sepsis-induced inflammation, administration of bacterial endotoxin (LPS) is often used to simulate sepsis in experimental animals. This study tests the hypothesis that LPS is a critical determinant of the hepatic microvascular dysfunction in mice made septic by cecal ligation and puncture (CLP). METHODS: Intravital videomicroscopy was used to quantify sinusoidal perfusion, and platelet and leukocyte adhesion in terminal hepatic venules (THV) and sinusoids in LPS-sensitive and LPS-insensitive mice subjected to CLP or LPS (i.p.). mRNA expression of TLR-2, TLR-4, MyD-88, and Ly-96 was also assessed. RESULTS: While LPS-sensitive mice responded to both CLP and LPS challenges with elevated leukocyte and platelet adhesion in THV and sinusoids, and a reduced sinusoidal perfusion density, LPS-insensitive mice exhibited comparable blood cell adhesion and sinusoidal malperfusion following CLP, but not LPS. Hepatic mRNA of MyD-88 and TLR-2 was elevated in the CLP and LPS groups. Endotoxin was not detectable in the blood of LPS-sensitive mice after CLP, but was elevated after LPS administration. CONCLUSIONS: These findings do not support a major role for LPS in the hepatic microvascular disturbances associated with polymicrobial sepsis.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blood Platelets - cytology
- Cell Adhesion - cytology
- Disease Models, Animal - cytology
- Endotoxins - blood
- Leukocytes - cytology
- Lipopolysaccharides - pharmacology
- Liver Circulation - drug effects
- Liver Diseases - etiology
- Mice - etiology
- Microcirculation - drug effects
- Microscopy, Video - drug effects
- Sepsis - etiology
- Find related publications in this database (Keywords)
- LPS
- platelets
- sepsis
- intravital fluorescence microscopy
- liver injury
- TLR-4