Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Salmhofer, W; Hermann, J; Joch, M; Kerl, H; Graninger, W.
High serum levels of antibodies against the recombinant 70 kDa ribonucleoprotein are useful for diagnosing mixed connective tissue disease.
J Eur Acad Dermatol Venereol. 2007; 21(8):1047-1053
Doi: 10.1111/j.1468-3083.2007.02163.x
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- Führende Autor*innen der Med Uni Graz
- Salmhofer Wolfgang
- Co-Autor*innen der Med Uni Graz
- Graninger Winfried
- Hermann Josef
- Joch Monika
- Kerl Helmut
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- Abstract:
- Anti-Sm antibodies and anti-RNP antibodies are considered to be diagnostic markers of systemic lupus erythematosus (SLE) and mixed connective tissue disease (MCTD). However, cross-reactivity between the antibodies diminishes their discriminating specificity between these diagnoses. We examined whether we could achieve better differentiation between these two disease entities using recombinant antigens to RNP70 and SmD and quantitative immunoassays. Sera from 51 patients with SLE and 10 patients with MCTD and from a control group of 59 patients were used in a cross-sectional setting. Semiquantitative ELISAs for the detection of antibodies to RNP-70, RNP-A, RNP-C, SmBB' and SmD were used and the results were compared to conventional ELISA tests using U(1)-snRNP and a mixture of SmBB' and SmD as antigenic substrates. Sera from MCTD patients showed higher levels of anti-RNP-70 antibodies than sera from SLE patients. Levels of anti-SmBB' or anti-SmD antibodies were not significantly different between SLE and MCTD sera. However, the presence of antibodies directed against SmD was more frequent in SLE. Our results suggest that the use of RNP-70 and SmD antigens may increase the practical value of immunoassays used to confirm a diagnosis of SLE or MCTD in patients with connective tissue disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Autoantigens - blood
- Autoantigens - immunology
- Biomarkers - blood
- Cross-Sectional Studies -
- Diagnosis, Differential -
- Enzyme-Linked Immunosorbent Assay -
- Female -
- Humans -
- Lupus Erythematosus, Systemic - diagnosis
- Lupus Erythematosus, Systemic - immunology
- Male -
- Middle Aged -
- Mixed Connective Tissue Disease - diagnosis
- Mixed Connective Tissue Disease - immunology
- Recombinant Proteins - blood
- Recombinant Proteins - immunology
- Ribonucleoproteins, Small Nuclear - blood
- Ribonucleoproteins, Small Nuclear - immunology
- Sensitivity and Specificity -
- Find related publications in this database (Keywords)
- antibody levels
- anti-RNP-70 antibodies
- anti-Sm antibodies
- anti-U-1-snRNP antibodies
- mixed connective tissue disease
- systemic lupus erythematosus