Medizinische Universität Graz - Research portal
Selected Publication:
Schuligoi, R; Amann, R; Angelberger, P; Peskar, BA.
Determination of nociceptin-like immunoreactivity in the rat dorsal spinal cord.
Neurosci Lett. 1997; 224(2):136-138
Doi: 10.1016%2FS0304-3940%2897%2913453-X
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- Leading authors Med Uni Graz
- Schuligoi Rufina
- Co-authors Med Uni Graz
- Amann Rainer
- Peskar Bernhard
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- Abstract:
- An endogenous ligand for the opioid receptor-like (ORL1) receptor has been characterized recently. This 17 amino acid peptide, nociceptin, has potent effects on pain processing in experimental animals. In the present study, we describe the development of a radio-immunoassay for nociceptin, suitable for quantification of immunoreactivity in the rat spinal cord. in the dorsal spinal cord we found 641 fmol nociceptin-like immunoreactivity (IR)/mg protein, while in the ventral spinal cord the amount was below the detection limit of the assay (11 fmol/mg protein). High pressure liquid chromatography (HPLC) showed that the immunoreactive material was heterogeneous with the major part eluting at the position of synthetic nociceptin. The presence of nociceptin in the dorsal spinal cord is compatible with its proposed involvement in modulating afferent transmission.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antibody Specificity -
- Chromatography, High Pressure Liquid -
- Nociceptors - chemistry
- Opioid Peptides - analysis
- Rabbits - analysis
- Radioimmunoassay - analysis
- Rats - analysis
- Receptors, Opioid - agonists
- Spinal Cord - chemistry
- Find related publications in this database (Keywords)
- Nociceptin
- Orphanin Fq
- Radioimmunoassay
- Rat
- Spinal Cord