Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schmidt, HH; Dyomin, VG; Palanisamy, N; Itoyama, T; Nanjangud, G; Pirc-Danoewinata, H; Haas, OA; Chaganti, RS.
Deregulation of the carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11) gene in a B-cell chronic lymphocytic leukemia with a t(12;14)(q23;q32).
Oncogene. 2004; 23(41):6991-6996
Doi: 10.1038/sj.onc.1207934
(- Case Report)
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- Führende Autor*innen der Med Uni Graz
- Schmidt Helmut
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- Abstract:
- The t(12;14)(q23;q32) breakpoints in a case of B-cell chronic lymphocytic leukemia (B-CLL) were mapped by fluorescence in situ hybridization (FISH) and Southern blot analysis and cloned using an IGH switch-gamma probe. The translocation affected a productively rearranged IGH allele and the carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11) locus at 12q23, with a reciprocal break in intron 2 of the CHST11 gene. CHST11 belongs to the HNK1 family of Golgi-associated sulfotransferases, a group of glycosaminoglycan-modifying enzymes, and is expressed mainly in the hematopoietic lineage. Northern Blot analysis of tumor RNA using CHST11-specific probes showed expression of two CHST11 forms of abnormal size. 5'- and 3'-Rapid Amplification of cDNA Ends (RACE) revealed IGH/CHST11 as well as CHST11/IGH fusion RNAs expressed from the der(14) and der(12) chromosomes. Both fusion species contained open reading frames making possible the translation of two truncated forms of CHST11 protein. The biological consequence of t(12;14)(q23;q32) in this case presumably is a disturbance of the cellular distribution of CHST11 leading to deregulation of a chondroitin-sulfate-dependent pathway specific to the hematopoietic lineage.
- Find related publications in this database (using NLM MeSH Indexing)
- Base Sequence -
- Chromosomes, Human, Pair 12 -
- Chromosomes, Human, Pair 14 -
- Gene Expression Regulation, Leukemic -
- Humans -
- Leukemia, Lymphocytic, Chronic, B-Cell - genetics
- Male -
- Middle Aged -
- Molecular Sequence Data -
- Sulfotransferases - genetics
- Translocation, Genetic -
- Find related publications in this database (Keywords)
- B-CLL
- translocation
- carbohydrate (chondroitin 4) sulfotransferase 11
- CHST11
- Golgi retention