Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Zorn-Pauly, K; Schaffer, P; Pelzmann, B; Bernhart, E; Lang, P; Koidl, B.
L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes.
PHYSIOL RES. 2004; 53(4): 369-377.
Doi: 10.33549/physiolres.930495
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- Führende Autor*innen der Med Uni Graz
- Koidl Bernd
- Zorn-Pauly Klaus
- Co-Autor*innen der Med Uni Graz
- Bernhart Eva Maria
- Lang Petra
- Pelzmann Brigitte
- Schaffer Peter
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- Abstract:
- The aim of this investigation was to study L-type and T-type Ca(2+) current (I(CaL) and I(CaT)) in short-term cultured adult guinea pig ventricular myocytes. The isolated myocytes were suspended in serum-supplemented medium up to 5 days. Using whole-cell patch clamp techniques ICaL and ICaT were studied by applying voltage protocols from different holding potentials (-40 and -90 mV). After 5 days in culture the myocytes still showed their typical rod shaped morphology but a decline in cell membrane capacitance (26 %). The peak density of ICaT was reduced significantly between day 0 (-1.6+/-0.37 pA/pF, n=9) and day 5 (-0.4+/-0.13 pA/pF, n=11), whereas peak ICaL density revealed no significant differences during culturing. The I(CaT)/I(CaL) ratio dropped from 0.13 at day 0 to 0.05 at day 5. Compared with day 0 I(CaL) the steady state inactivation curve of day 1, day 3 and day 5 myocytes was slightly shifted to more negative potentials. Our data indicate that guinea pig ventricular L-type and T-type Ca(2+) channels are differently regulated in culture.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Calcium Channels, L-Type - physiology
- Calcium Channels, T-Type - physiology
- Cells, Cultured -
- Guinea Pigs -
- Heart Ventricles - cytology
- Myocardium - metabolism
- Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Myocytes, Cardiac - physiology
- Ventricular Function -
- Find related publications in this database (Keywords)
- ventricular myocytes
- short-term culture
- L-type Ca2+ channel
- T-type Ca2+ channel