Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wultsch, T; Painsipp, E; Shahbazian, A; Mitrovic, M; Edelsbrunner, M; Lazdunski, M; Waldmann, R; Holzer, P.
Deletion of the acid-sensing ion channel ASIC3 prevents gastritis-induced acid hyperresponsiveness of the stomach-brainstem axis.
Pain. 2008; 134(3): 245-253.
Doi: 10.1016/j.pain.2007.04.025
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- Führende Autor*innen der Med Uni Graz
- Holzer Peter
- Co-Autor*innen der Med Uni Graz
- Edelsbrunner Martin Erich
- Mitrovic Martina
- Singewald Evelin
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- Abstract:
- Gastric acid challenge of the rat and mouse stomach is signalled to the brainstem as revealed by expression of c-Fos. The molecular sensors relevant to the detection of gastric mucosal acidosis are not known. Since the acid-sensing ion channels ASIC2 and ASIC3 are expressed by primary afferent neurons, we examined whether knockout of the ASIC2 or ASIC3 gene modifies afferent signalling of a gastric acid insult in the normal and inflamed stomach. The stomach of conscious mice (C57BL/6) was challenged with intragastric HCl; two hours later the activation of neurons in the nucleus tractus solitarii (NTS) of the brainstem was visualized by c-Fos immunocytochemistry. Mild gastritis was induced by addition of iodoacetamide (0.1%) to the drinking water for 7 days. Exposure of the gastric mucosa to HCl (0.25M) caused a 3-fold increase in the number of c-Fos-positive neurons in the NTS. This afferent input to the NTS remained unchanged by ASIC3 knockout, whereas ASIC2 knockout augmented the c-Fos response to gastric HCl challenge by 33% (P<0.01). Pretreatment of wild-type mice with iodoacetamide induced mild gastritis, as revealed by increased myeloperoxidase activity, and enhanced the number of NTS neurons responding to gastric HCl challenge by 41% (P<0.01). This gastric acid hyperresponsiveness was absent in ASIC3 knockout mice but fully preserved in ASIC2 knockout mice. The current data indicate that ASIC3 plays a major role in the acid hyperresponsiveness associated with experimental gastritis. In contrast, ASIC2 appears to dampen acid-evoked input from the stomach to the NTS.
- Find related publications in this database (using NLM MeSH Indexing)
- Afferent Pathways - physiopathology
- Animals -
- Brain Stem - drug effects
- Gastric Acid - metabolism
- Gastritis - chemically induced
- Gene Deletion -
- Hydrochloric Acid -
- Hypersensitivity - metabolism
- Mice -
- Mice, Inbred C57BL -
- Mice, Transgenic -
- Proto-Oncogene Proteins c-fos - metabolism
- Sodium Channels - genetics
- Stomach - drug effects
- Find related publications in this database (Keywords)
- acid-sensing ion channels
- gastric acid hyperresponsiveness
- gastritis
- expression of c-Fos
- nucleus of the solitary tract
- vagal afferent neurons