Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lindschinger, M; Nadlinger, K; Adelwöhrer, N; Holweg, K; Wögerbauer, M; Birkmayer, J; Smolle, KH; Wonisch, W.
Oxidative stress: potential of distinct peroxide determination systems.
CLIN CHEM LAB MED. 2004; 42(8): 907-914.
Doi: 10.1515/CCLM.2004.147
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Smolle Karl-Heinz
- Wonisch Willibald
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- Abstract:
- When reactive oxygen species attack biological structures, peroxides, which are short-lived oxidative intermediates, are generated. We evaluated the potential of two different, commercially available peroxide activity assays (Pox-Act and d-ROMS) to see whether the results were associated with the clinical condition of subjects who were participating in a routine health care program. Furthermore, we determined the total antioxidant status (TAS) and the titer of autoantibodies against oxidized low-density lipoprotein (oLAb) to verify the hydroperoxide measurements. Subjects with medical conditions (hereafter referred to as patients) had significantly increased serum peroxide levels compared to healthy subjects. The d-ROMS kit indicated that 86% of subjects had an increased level of total peroxides. Although the assays had a significant correlation (p<0.001), 34% of the subjects had an increased total peroxide concentration in the Pox-Act assay that was clearly associated with clinical symptoms. Furthermore, the sensitivity of the Pox-Act assay was 35 times higher than that of the d-ROMS kit. In subjects with medical conditions, there was a trend toward a decreased TAS and a slightly increased oLAb titer in comparison to healthy subjects, but this was not statistically significant. The Pox-Act assay seems to be a valuable tool for the determination of total peroxides, while the results from the d-ROMS kit should be considered with caution.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Antioxidants - analysis Antioxidants - metabolism
- Autoantibodies - blood Autoantibodies - immunology
- Blood Chemical Analysis - methods
- Female -
- Humans -
- Lipid Peroxides - analysis Lipid Peroxides - blood
- Lipoproteins, LDL - blood Lipoproteins, LDL - immunology Lipoproteins, LDL - metabolism
- Male -
- Oxidative Stress - physiology
- Predictive Value of Tests -
- Reproducibility of Results -
- Sensitivity and Specificity -
- Find related publications in this database (Keywords)
- antioxidants
- lipid peroxidation
- oxidative stress
- oxidative stress measurement
- ox-LDL antibodies
- peroxides