Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Oettl, K; Greilberger, J; Dikalov, S; Reibnegger, G.
Interference of 7,8-dihydroneopterin with peroxynitrite-mediated reactions.
BIOCHEM BIOPHYS RES COMMUN. 2004; 321: 379-385.
Doi: 10.1016/j.bbrc.2004.06.157
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Öttl Karl
- Co-Autor*innen der Med Uni Graz
- Greilberger Joachim
- Reibnegger Gilbert
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- Abstract:
- By in vitro studies 7,8-dihydroneopterin, which is secreted by macrophages stimulated by interferon-gamma, was reported to be a radical scavenger as well as a prooxidative agent depending on the experimental settings. In this study, we investigated the interference of 7,8-dihydroneopterin with peroxynitrite mediated reactions by different analytical procedures. Luminol chemiluminescence and oxidation of the spin probe 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine induced by peroxynitrite were inhibited by 7,8-dihydroneopterin. On the other hand, we found that 7,8-dihydroneopterin very efficiently inhibits nitration of tyrosine by peroxynitrite. Hydroxylation, however, was rather enhanced than inhibited, suggesting that 7,8-dihydroneopterin reacts in quite different manner with the intermediates generated from peroxynitrite. We provide the first evidence that a pterin radical is formed from a dihydropterin using EPR spectroscopy and 2,2,4-trimethyl-2H-imidazole-1-oxide as a spin trap. We conclude that 7,8-dihydroneopterin while being a weak scavenger of superoxide acts as a very efficient inhibitor of tyrosine nitration induced by peroxynitrite.
- Find related publications in this database (using NLM MeSH Indexing)
- Chemiluminescent Measurements -
- Electron Spin Resonance Spectroscopy -
- Free Radical Scavengers - metabolism
- Hydroxylation - metabolism
- Molsidomine - analogs and derivatives
- Neopterin - analogs and derivatives
- Oxidation-Reduction - analogs and derivatives
- Peroxynitrous Acid - metabolism
- Piperidines - metabolism
- Salicylic Acid - metabolism
- Spectrometry, Fluorescence - metabolism
- Superoxides - metabolism
- Tyrosine - chemistry
- Find related publications in this database (Keywords)
- pterins
- free radicals
- antioxidants
- spin trap
- spin probe
- EPR-spectroscopy
- nitration
- hydroxylation