Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Barth, A; Guzman, R; Andres, RH; Mordasini, P; Barth, L; Widmer, HR.
Experimental intracerebral hematoma in the rat.
Restor Neurol Neurosci. 2007; 25(1):1-7
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Barth Alain
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Abstract:
- PURPOSE: Current models of intracerebral hematoma are difficult to use for neurotransplantation studies because of high mortality and important variations of morphology, size and location of blood deposits. We propose a modification of the autologous blood infusion technique in rats to reduce these limitations. METHODS: The modification consisted in a mechanical microlesion preceding blood infusion. A canula was stereotactically introduced into the striatum of adult rats. Subsequently, a parenchyma lesion was created by a rotating microcatheter coaxially inserted through the canula, followed by slow infusion of 30 mul autologous blood during 5 minutes. Controls included canula insertion only and canula + microlesion. Hematoma volume/morphology were quantified and the animals behaviorally analysed using standardized tests. RESULTS: Surgical mortality was 0/54 rats. One animal died during follow-up. Hematoma volume was constant and significantly higher (15.20 +/- 0.60 mm;3) than control lesions (canula: 0.11 +/- 0.01 mm;3; canula + trauma: 0.51 +/- 0.01 mm;3). Hematoma edges were sharply delineated and the perihematomal region histologically preserved. Rats with hematoma showed initially a reduced spontaneous rotational behaviour. They also showed persisting deficits of forelimb placing ability. CONCLUSIONS: The advantages of this model include a systematic control of all steps of hematoma production, high reproducibility of volume, size, and location of blood deposits, preservation of perihematomal brain tissue, and quantifiable neurological deficits.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Behavior, Animal -
- Blood Transfusion, Autologous - adverse effects
- Brain - metabolism
- Cerebral Hemorrhage - mortality
- Disease Models, Animal - mortality
- Dopamine and cAMP-Regulated Phosphoprotein 32 - metabolism
- Hematoma - etiology
- Magnetic Resonance Imaging - methods
- Male - methods
- Rats - methods
- Rats, Wistar - methods
- Rotation - methods
- Time Factors - methods
- Find related publications in this database (Keywords)
- hemorrhagic stroke
- intracerebral
- hematoma
- rat