Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bálint, Z; Krizbai, IA; Wilhelm, I; Farkas, AE; Párducz, A; Szegletes, Z; Váró, G.
Changes induced by hyperosmotic mannitol in cerebral endothelial cells: an atomic force microscopic study.
Eur Biophys J. 2007; 36(2):113-120
Doi: 10.1007/s00249-006-0112-4
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Balint Zoltan
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Understanding the reaction of living cells in response to different extracellular stimuli, such as hyperosmotic stress, is of primordial importance. Mannitol, a cell-impermeable non-toxic alcohol, has been used successfully for reversible opening of the blood-brain barrier in hyperosmotic concentrations. In this study we analyzed the effect of hyperosmotic mannitol on the shape and surface structure of living cerebral endothelial cells by atomic force microscope imaging technique. Addition of clinically relevant concentrations of mannitol to the culture medium of the confluent cells induced a decrease of about 40% in the observed height of the cells. This change was consistent both at the nuclear and peripheral region of the cells. After mannitol treatment even a close examination of the contact surface between the cells did not reveal gap between them. We could observe the appearance of surface protrusions of about 100 nm. By force measurements the elasticity of the cells were estimated. While the Young's modulus of the control cells appeared to be 8.04 +/- 0.12 kPa, for the mannitol-treated cells it decreased to an estimated value of 0.93 +/- 0.04 kPa which points to large structural changes inside the cell.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain - cytology Brain - drug effects Brain - physiology
- Cell Size - drug effects
- Cells, Cultured -
- Dose-Response Relationship, Drug -
- Elasticity -
- Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - physiology
- Mannitol - administration and dosage
- Membrane Fluidity - drug effects Membrane Fluidity - physiology
- Microscopy, Atomic Force - methods
- Osmotic Pressure - drug effects
- Rats -
- Stress, Mechanical -
- Find related publications in this database (Keywords)
- blood-brain barrier
- cell imaging
- cytoskeleton
- force measurement
- Young's modulus