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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Laczkó, I; Váró, G; Bottka, S; Bálint, Z; Illyés, E; Vass, E; Bertrand, JR; Malvy, C; Hollósi, M.
N-terminal acylation of the SV40 nuclear localization signal peptide enhances its oligonucleotide binding and membrane translocation efficiencies.
Arch Biochem Biophys. 2006; 454(2):146-154
Doi: 10.1016/j.abb.2006.08.015
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- Co-authors Med Uni Graz
- Balint Zoltan
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- Abstract:
- Octanoyl and palmitoyl groups were coupled to the N-terminus of an analog of the SV40 nuclear localization signal peptide, SV126-133(Ser128), to study the effect of the fatty acid chain length on the complex formation with a single-stranded antisense oligodeoxynucleotide (ODN) and on the cellular uptake of the complex. The strongest binding affinity was observed for the palmitoylated peptide, indicating the better accessibility of the positively charged lysyl and arginyl side-chains to the phosphate groups due to the turn structures stabilized by the palmitoyl group. On increase of the peptide to ODN molar ratio (rM), gradual unstacking of the bases was observed, the maximal rate being reached at rM=10. At rM>10 restacking of the nucleotide bases was detected and the ODN was completely encapsulated in a liposome-like structure made up of palmitoylated peptides. Cell translocation experiments revealed a highly efficient cell transport of the ODN by palmitoylated SV40 peptide at rM>10.
- Find related publications in this database (using NLM MeSH Indexing)
- Acylation -
- Animals -
- Biological Transport -
- Cell Membrane Permeability -
- Circular Dichroism -
- Drug Carriers -
- Gene Transfer Techniques -
- Green Fluorescent Proteins - genetics
- Mice -
- Microscopy, Atomic Force -
- Molecular Conformation -
- NIH 3T3 Cells -
- Octanoic Acids - chemistry
- Oligodeoxyribonucleotides, Antisense - administration and dosage Oligodeoxyribonucleotides, Antisense - metabolism Oligodeoxyribonucleotides, Antisense - pharmacology
- Oligopeptides - chemistry
- Oncogene Proteins, Fusion - genetics
- Palmitic Acids - chemistry
- Proto-Oncogene Protein c-fli-1 - genetics
- Spectroscopy, Fourier Transform Infrared -
- Find related publications in this database (Keywords)
- antisense oligonucleotide
- acylated peptides
- attenuated total reflection FTIR
- atomic force microscopy
- cell translocation
- circular dichroism
- epifluorescence
- nuclear localization signal peptide