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SHR Neuro Cancer Cardio Lipid Metab Microb
Cvirn, G; Gallistl, S; Kutschera, J; Wagner, T; Ferstl, U; Jurgens, G; Koestenberger, M.
Collagen/endogenous thrombin-induced platelet aggregation in whole blood samples.
Blood Coagul Fibrinolysis. 2007; 18(6): 585-588.
Doi: 10.1097/MBC.0b013e328285d832
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- Leading authors Med Uni Graz
- Cvirn Gerhard
- Co-authors Med Uni Graz
- Ferstl Ulrika
- Gallistl Siegfried
- Jürgens Günther
- Koestenberger Martin
- Wagner Thomas
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- Abstract:
- The aim of the study was to investigate the individual and combined effects of collagen (3.5 microg/ml) and endogenously generated thrombin (due to addition of 0.35 pmol/l tissue factor) on platelet aggregation in the physiological environment of whole blood by means of the impedance method. Lag times were significantly shorter when a combination of collagen and endogenous thrombin was used to provoke platelet aggregation (41.9 +/- 16.3 s) compared with collagen (173.8 +/- 52.1 s, P < 0.0001) or endogenous thrombin (94.3 +/- 43.6 s, P < 0.001). Amplitudes and slopes were the lowest in collagen-induced experiments (2.83 +/- 1.59 Omega and 1.79 +/- 0.45 Omega/min, respectively), whereas they were approximately the same in endogenous thrombin-induced experiments whether collagen was present or not (13.7 +/- 3.1 versus 11.2 +/- 4.0 Omega and 6.3 +/- 2.8 versus 5.6 +/- 2.3 Omega/min, respectively). No synergistic effect of collagen and endogenous thrombin on the clot formation process was observed by means of thrombelastometry. Moreover, thrombin potentials in tissue factor-activated plasma samples were approximately the same whether collagen was present or not (834 +/- 67 versus 809 +/- 63 nmol/l.min). In conclusion, endogenously generated thrombin is a potent platelet agonist in whole blood, and a combination of collagen and endogenous thrombin synergistically shortens the lag time until the onset of platelet aggregation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Blood Coagulation - physiology
- Collagen - physiology
- Humans - physiology
- Male - physiology
- Platelet Aggregation - physiology
- Thrombelastography - methods
- Thrombin - physiology
- Find related publications in this database (Keywords)
- collagen
- endogenous thrombin
- thrombelastography
- thrombin generation
- whole blood aggregometry