Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kucera, E; Speiser, P; Gnant, M; Szabo, L; Samonigg, H; Hausmaninger, H; Mittlböck, M; Fridrik, M; Seifert, M; Kubista, E; Reiner, A; Zeillinger, R; Jakesz, R.
Prognostic significance of mutations in the p53 gene, particularly in the zinc-binding domains, in lymph node- and steroid receptor positive breast cancer patients. Austrian Breast Cancer Study Group.
Eur J Cancer. 1999; 35(3):398-405
Doi: 10.1016/S0959-8049(98)00400-6
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- Co-Autor*innen der Med Uni Graz
- Samonigg Hellmut
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- Abstract:
- The aim of our study was to evaluate if p53 mutations, especially those in the L2/L3 domains of the p53 gene, add prognostic information for node-positive and steroid receptor positive breast cancer patients. Two hundred and five tumour samples from a randomised clinical trial of 596 lymph node- and steroid receptor positive breast cancer patients were included. All patients had been randomly allocated to receive 20 mg of adjuvant tamoxifen (TAM) daily for 2 years or TAM plus one cycle of low-dose, short-term chemotherapy. For detection of p53 mutations we used in vitro amplification by polymerase chain reaction and consecutively performed temperature gradient gel electrophoresis (PCR-TGGE) and direct sequencing. We found p53 mutations in 42/205 (20%) cases: 16/42 (38%) p53 mutations occurred within the L2/L3 domains of the p53 gene, and 26/42 (62%) outside the L2/L3 domains. p53 mutation served as a statistically significant parameter in predicting disease-free survival in univariate (P = 0.02) and multivariate (P = 0.009) analysis. For overall survival, no significant differences were observed. Patients with tumours that had p53 mutations within the L2/L3 domains of the gene showed no significant difference to those with mutations outside the L2/L3 domains for disease-free survival. For overall survival, mutations in the L2/L3 domains showed a marginally significant difference (P = 0.05) in multivariate analysis, but not in univariate analysis (P = 0.13). We conclude that mutation in the L2/L3 domains of the p53 gene is not an independent prognostic indicator of disease outcome for patients suffering from breast cancer with lymph node metastases and positive steroid receptors.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Breast Neoplasms - drug therapy Breast Neoplasms - genetics Breast Neoplasms - metabolism
- Disease-Free Survival -
- Female -
- Genes, p53 - genetics
- Humans -
- Lymphatic Metastasis -
- Middle Aged -
- Mutation - genetics
- Polymerase Chain Reaction - methods
- Prognosis -
- Receptors, Steroid - metabolism
- Survival Analysis -
- Tamoxifen - therapeutic use
- Zinc - metabolism
- Find related publications in this database (Keywords)
- breast cancer
- lymph node-positive
- steroid receptor positive
- prognosis
- p53 mutations
- L2/L3 domains
- PCR
- temperature gradient gel electrophoresis
- low-dose chemotherapy