Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Reti, NG; Lappas, M; Huppertz, B; Riley, C; Wlodek, ME; Henschke, P; Permezel, M; Rice, GE.
Effect of high oxygen on placental function in short-term explant cultures.
Cell Tissue Res. 2007; 328(3):607-616
Doi: 10.1007/s00441-006-0375-1
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- Co-authors Med Uni Graz
- Huppertz Berthold
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- Abstract:
- Ex situ culture of human gestational tissues has been routinely used as a model to investigate tissue function. The objective of this study was to determine the effect of varying oxygen concentrations on human term placental explants over a 24-h time period. Specifically, the effect of incubating placental explants in oxygen concentrations of 8%, 21% or 95% on tissue viability, metabolism and cell death was measured by assessing glucose consumption, lactate production, release of lactate dehydrogenase, parathyroid hormone-related protein (PTHrP), tumour necrosis factor-alpha (TNF-alpha) and 8-isoprostane, immunoreactivity for cleaved-caspase-9 and immunohistochemistry for the caspase-3-cleaved cytokeratin-18 neoepitope, M30. Exposure to higher oxygen concentrations significantly increased the rates of glucose consumption and lactate production. Apoptosis was significantly increased under conditions of higher oxygen as evidenced by increased M30 in placental explant sections. Similarly, hyperoxia significantly increased the releases of PTHrP, TNF-alpha and 8-isoprostane. Thus, incubation of placental explants with oxygen concentrations of 95% and, to a lesser extent, 21% oxygen was associated with the modulation of multiple cellular response pathways including those associated with tissue viability and cell death. These data are consistent with the hypothesis that hyperoxia activates pathways and mechanisms involved in cellular metabolism, necrosis and apoptosis, thereby shifting the balance from a steady state towards cell death.
- Find related publications in this database (using NLM MeSH Indexing)
- Apoptosis - drug effects
- Cell Membrane - drug effects
- Cells, Cultured - drug effects
- Dinoprost - analogs and derivatives
- Female - analogs and derivatives
- Humans - analogs and derivatives
- Organ Culture Techniques - analogs and derivatives
- Oxygen - pharmacology
- Parathyroid Hormone-Related Protein - secretion
- Placenta - drug effects
- Pregnancy - drug effects
- Time Factors - drug effects
- Tumor Necrosis Factor-alpha - secretion
- Find related publications in this database (Keywords)
- term placental explants
- short-term cultures
- oxidant stress
- metabolite activity
- apoptosis
- human