Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pachler, C; Ikeoka, D; Plank, J; Weinhandl, H; Suppan, M; Mader, JK; Bodenlenz, M; Regittnig, W; Mangge, H; Pieber, TR; Ellmerer, M.
Subcutaneous adipose tissue exerts proinflammatory cytokines after minimal trauma in humans.
Am J Physiol Endocrinol Metab. 2007; 293(3):E690-E696
Doi: 10.1152/ajpendo.00034.2007
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- Führende Autor*innen der Med Uni Graz
- Ellmerer Martin
- Pachler Christoph
- Co-Autor*innen der Med Uni Graz
- Ikeoka Dimas
- Mader Julia
- Mangge Harald
- Pieber Thomas
- Regittnig Werner
- Weinhandl Heinz
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- Abstract:
- Inflammatory cytokines released from adipose tissue play an important role in different pathological processes. In the present study, we investigated the inflammatory cytokine response of human subcutaneous adipose tissue (SAT) by applying the open-flow microperfusion technique. Four standard 18-gauge microperfusion catheters were inserted into periumbilical SAT of eight healthy male volunteers [29 +/- 3 yr, BMI 24.3 +/- 1.9 (mean +/- SD)]. SAT probe effluents were collected at 60-min intervals for 8 h after catheter insertion. Different perfusion fluids were used to measure the local effect of insulin and/or glucose on the cytokine response. SAT probe effluents were analyzed for IL-1beta, IL-6, CXCL8 (IL-8), and TNF-alpha. SAT concentrations of IL-1beta increased 100-fold from 1.0 +/- 0.2 pg/ml (mean +/- SE) to 101.5 +/- 23.2 pg/ml (P < 0.001) after 8 h. A 130-fold increase was observed for CXCL8, from 49 +/- 29 to 6,554 +/- 1,713 pg/ml (P < 0.001). Furthermore, a 20-fold increase of IL-6 was observed within the first 5 h (from 159 +/- 123 to 3,554 +/- 394 pg/ml; P < 0.001), and a significant decline to 2,154 +/- 216 pg/ml (P < 0.01) was seen thereafter. Finally, TNF-alpha increased from 1.4 +/- 0.6 to 2.5 +/- 0.5 pg/ml (P < 0.05) in hour 2 and remained stable thereafter. Local administration of insulin exerted a stimulatory effect on the inflammatory response of IL-6. In conclusion, SAT exerts a highly reproducible and consistent proinflammatory cytokine response after minimally invasive trauma caused by the insertion of a catheter in humans.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipose Tissue - immunology
- Adult -
- Cytokines - immunology
- Humans -
- Immunologic Factors - immunology
- Inflammation - chemically induced Inflammation - immunology
- Insulin -
- Male -
- Skin - immunology Skin - injuries
- Wounds and Injuries - immunology