Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Rohde, E; Bartmann, C; Schallmoser, K; Reinisch, A; Lanzer, G; Linkesch, W; Guelly, C; Strunk, D.
Immune cells mimic the morphology of endothelial progenitor colonies in vitro.
Stem Cells. 2007; 25(7): 1746-1752.
Doi: 10.1634/stemcells.2006-0833
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- Führende Autor*innen der Med Uni Graz
- Rohde Eva
- Strunk Dirk
- Co-Autor*innen der Med Uni Graz
- Gülly Christian
- Lanzer Gerhard
- Linkesch Werner
- Reinisch Andreas
- Schallmoser Katharina
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- Abstract:
- Endothelial progenitor cells (EPC) are considered powerful biologic markers for vascular function and cardiovascular risk, predicting events and death from cardiovascular causes. Colony-forming units of endothelial progenitor cells (CFU-EC) are used to quantify EPC circulating in human peripheral blood. The mechanisms underlying colony formation and the nature of the contributing cells are not clear. We performed subtractive CFU-EC analyses to determine the impact of various blood cell types and kinetics of protein and gene expression during colony formation. We found that CFU-EC mainly comprise T cells and monocytes admixed with B cells and natural killer cells. The combination of purified T cells and monocytes formed CFU-EC structures. The lack of colonies after depletion or functional ablation of T cells or monocytes was contrasted with effective CFU-EC formation in the absence of CD34+ cells. Microarray analyses revealed activation of immune function-related biological processes without changes in angiogenesis-related processes during colony formation. In concordance with a regenerative function, soluble factors derived from CFU-EC cultures supported vascular network formation in vitro. Recognizing CFU-EC formation as the result of a functional cross between T cells and monocytes shifts expectations of vascular regenerative medicine. Our data support the move from a view of circulating EPC toward models that include a role for immune cells in vascular regeneration. Disclosure of potential conflicts of interest is found at the end of this article.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Antigens, CD34 - metabolism
- Blood Vessels - metabolism
- Endothelial Cells - cytology
- Genome, Human - genetics
- Humans - genetics
- Lymphocyte Activation - genetics
- Lymphocytes - cytology
- Male - cytology
- Middle Aged - cytology
- Monocytes - cytology
- Oligonucleotide Array Sequence Analysis - cytology
- Stem Cells - cytology
- Find related publications in this database (Keywords)
- endothelial progenitor cells
- colony-forming units of endothelial progenitor cells
- vascular regeneration
- regenerative medicine
- immunity