Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Stelzl, E; van der Meer, C; Gouw, R; Beld, M; Grahovac, M; Marth, E; Kessler, HH.
Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays.
CLIN CHEM LAB MED. 2007; 45(2): 167-170.
Doi: 10.1515/CCLM.2007.043
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Kessler Harald
- Stelzl Evelyn
- Co-authors Med Uni Graz
- Marth Egon
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- Abstract:
- BACKGROUND: Hepatitis C virus (HCV) genotyping and accurate subtyping is becoming increasingly relevant to epidemiological studies, clinical management, pathogenicity, and vaccine development. METHODS: The TRUGENE HCV 5'NC Genotyping Kit, the new VERSANT HCV Genotype 2.0 Assay (LiPA), and a new laboratory-developed HCV NS5b sequencing assay designed for automated sequencing of the HCV NS5b region were used. Clinical samples and a molecular diagnostics HCV genotyping proficiency program panel were used to determine accuracy and differentiate performance characteristics of the three methods. RESULTS: All amplified samples from among the members of a HCV genotyping proficiency program panel that contained a single HCV genotype were subtyped correctly using all three HCV genotyping assays. With the TRUGENE HCV 5'NC Genotyping Kit, the HCV subtype was determined in 357 of 441 of routine clinical samples. When the 84 samples with only genotype results were retested with the VERSANT HCV Genotype 2.0 Assay (LiPA), 61 could be further subtyped accurately. With the new laboratory-developed HCV NS5b sequencing assay, all 84 could be subtyped accurately. CONCLUSIONS: The two new methods show advantages over the routinely used TRUGENE HCV 5'NC Genotyping Kit in terms of genotyping and subtyping accuracy by utilizing part of the HCV core region and NS5b region, respectively.
- Find related publications in this database (using NLM MeSH Indexing)
- Genotype -
- Hepacivirus - isolation and purification
- Nucleic Acid Hybridization - methods
- Reagent Kits, Diagnostic - standards
- Sequence Analysis, DNA - methods
- Viral Nonstructural Proteins - genetics
- Find related publications in this database (Keywords)
- hepatitis C virus
- NS5b
- reverse hybridization
- sequencing
- subtype