Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Geigl, JB; Uhrig, S; Speicher, MR.
Multiplex-fluorescence in situ hybridization for chromosome karyotyping.
Nat Protoc. 2006; 1(3): 1172-1184.
Doi: 10.1038/nprot.2006.160
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Geigl Jochen Bernd
- Speicher Michael
- Co-Autor*innen der Med Uni Graz
- Uhrig Sabine
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- Abstract:
- Multiplex-fluorescence in situ hybridization (M-FISH) was initially developed to stain human chromosomes--the 22 autosomes and X and Y sex chromosomes--with uniquely distinctive colors to facilitate karyotyping. The characteristic spectral signatures of all different combinations of fluorochromes are determined by multichannel image-analysis methods. Advantages of M-FISH include rapid analysis of metaphase spreads, even in complex cases with multiple chromosomal rearrangements, and identification of marker chromosomes. The M-FISH technology has been extended to other species, such as the mouse. Furthermore, in addition to painting probes, the method has been used with a variety of region-specific probes. M-FISH has even recently been used for 3D studies to analyze the distribution of human chromosomes in intact and preserved interphase nuclei. Hence, M-FISH has evolved into an essential tool for both clinical diagnostics and basic research. In this protocol, we describe how to use M-FISH to karyotype chromosomes, a procedure that takes approximately 14 d if new M-FISH probes have to be generated and 3 d if the M-FISH probes are ready to use.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Chromosomes - genetics
- Fluorescent Dyes - analysis
- Humans - analysis
- In Situ Hybridization, Fluorescence - methods
- Karyotyping - methods
- Mice - methods