Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kulterer, B; Friedl, G; Jandrositz, A; Sanchez-Cabo, F; Prokesch, A; Paar, C; Scheideler, M; Windhager, R; Preisegger, KH; Trajanoski, Z.
Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation.
BMC Genomics. 2007; 8(5): 70-70.
Doi: 10.1186/1471-2164-8-70
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- Co-Autor*innen der Med Uni Graz
- Friedl Gerald
- Prokesch Andreas
- Windhager Reinhard
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- Abstract:
- Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-beta2 and BMPs. With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications.
- Find related publications in this database (using NLM MeSH Indexing)
- Bone Marrow Cells - cytology Bone Marrow Cells - metabolism
- Cell Differentiation - genetics
- Cluster Analysis -
- Colony-Forming Units Assay -
- Gene Expression Profiling - methods
- Humans -
- Inhibitor of Differentiation Proteins - genetics
- Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism
- Oligonucleotide Array Sequence Analysis - methods
- Osteoblasts - cytology Osteoblasts - metabolism
- Osteogenesis - genetics
- Reverse Transcriptase Polymerase Chain Reaction -
- alpha-Crystallin B Chain - genetics