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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Michelin, BD; Muller, Z; Stelzl, E; Marth, E; Kessler, HH.
Evaluation of the Abbott RealTime HCV assay for quantitative detection of hepatitis C virus RNA.
J CLIN VIROL. 2007; 38(2): 96-100.
Doi: 10.1016/j.jcv.2006.11.007
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Kessler Harald
- Marth Egon
- Stelzl Evelyn
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- Abstract:
- BACKGROUND: The Abbott RealTime HCV assay for quantitative detection of HCV RNA has recently been introduced. OBJECTIVES: In this study, the performance of the Abbott RealTime HCV assay was evaluated and compared to the COBAS AmpliPrep/COBAS TaqMan HCV test. STUDY DESIGN: Accuracy, linearity, interassay and intra-assay variations were determined, and a total of 243 routine clinical samples were investigated. RESULTS: When accuracy of the new assay was tested, the majority of results were found to be within +/-0.5 log(10) unit of the results obtained by reference laboratories. Determination of linearity resulted in a quasilinear curve up to 1.0 x 10(6)IU/ml. The interassay variation ranged from 15% to 32%, and the intra-assay variation ranged from 5% to 8%. When clinical samples were tested by the Abbott RealTime HCV assay and the results were compared with those obtained by the COBAS AmpliPrep/COBAS TaqMan HCV test, the results for 93% of all samples with positive results by both tests were found to be within +/-1.0 log(10) unit. The viral loads for all patients measured by the Abbott and Roche assays showed a high correlation (R(2)=0.93); quantitative results obtained by the Abbott assay were found to be lower than those obtained by the Roche assay. CONCLUSIONS: The Abbott RealTime HCV assay proved to be suitable for use in the routine diagnostic laboratory. The time to results was similar for both of the assays.
- Find related publications in this database (using NLM MeSH Indexing)
- Hepacivirus - genetics
- Hepatitis C - virology
- Humans -
- Polymerase Chain Reaction - methods
- RNA, Viral - analysis RNA, Viral - genetics
- Find related publications in this database (Keywords)
- hepatitis C virus
- real-time PCR
- automation
- Abbott RealTime HCV assay
- COBAS AmpliPrep
- COBAS TaqMan