Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
März, W; Gross, W.
Analysis of plasma lipoproteins by ultracentrifugation in a new fixed angle rotor: evaluation of a phosphotungstic acid/MgCl2 precipitation and a quantitative lipoprotein electrophoresis assay.
Clin Chim Acta. 1986; 160(1):1-18
Doi: 10.1016/0009-8981(86)90330-X
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- Führende Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- A routine procedure for the ultracentrifugal analysis of human plasma lipoproteins by means of a fixed angle rotor was developed. It was applied as reference method for the evaluation of two widely used procedures of lipoprotein analysis, i.e. measurement of HDL-cholesterol after precipitation of the apolipoprotein B containing lipoproteins with a new phosphotungstic acid/MgCl2 reagent, and a quantitative lipoprotein electrophoresis system. A new statistical approach to the multivariate comparison of analytical methods, the linear structural relationship model, has been applied. The HDL-cholesterol levels measured after phosphotungstic acid/MgCl2 precipitation agreed well to those determined by ultracentrifugation, whereas electrophoretically quantified alpha-lipoprotein-cholesterol exceeded the ultracentrifugal HDL-cholesterol. The Friedewald formula obviously underestimated LDL-cholesterol due to an overestimation of VLDL-cholesterol, whereas the electrophoretically quantified pre-beta- and beta-lipoprotein-cholesterol levels fairly coincided with the respective ultracentrifugal measurements. The inter-assay reproducibility of the HDL-cholesterol determination after phosphotungstic acid/MgCl2 precipitation and subsequent LDL-cholesterol quantification according to Friedewald was statistically equivalent to that of ultracentrifugation, whereas the quantitative lipoprotein electrophoresis proved less precise.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cholesterol - blood
- Cholesterol, HDL - blood
- Cholesterol, LDL - blood
- Cholesterol, VLDL - blood
- Electrophoresis - blood
- Female - blood
- Lipoproteins - blood
- Lipoproteins, VLDL - blood
- Magnesium - blood
- Magnesium Chloride - blood
- Phosphotungstic Acid - blood
- Precipitation - blood
- Ultracentrifugation - blood