Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Chen, M; Maerz, W; Manfras, BJ; Kuehnl, P; Usadel, KH; Boehm, BO.
Rapid and simple subtyping of the HLA-DRB3 gene in Graves' disease by using temperature-gradient gel electrophoresis.
Hum Immunol. 1993; 36(3):199-203
Doi: 10.1016/0198-8859(93)90126-L
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- Co-Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- A HLA-DRB3-subtyping system that uses TGGE for analyzing DRB3 alleles was developed. The polymorphic second exon of the HLA-DRB3 gene was amplified from four homozygous typing cell lines, 223 healthy individuals, and 102 patients with Graves' disease by using the PCR. The PCR products were electrophoresed in a temperature gradient from 35 degrees C to 70 degrees C, and the resulting fragments were visualized by silver staining. Four DRB3 alleles (HLA-DRB3*0101, *0201, *0202, and *0301) were distinguished from one another by the migration of the corresponding homoduplex with the exception that DRB3*0201 was indistinguishable from DRB3*0202. The latter two alleles, however, were resolved by the artificial heteroduplexing approach. Arginine in position 74 of the DRB3 gene product (i.e., HLA-DRB3*0101) was significantly more frequent in Graves' patients than in controls. The relative risk conferred by the presence of the DRB3*0101 was 15.8 (p < or = 0.001). The presence of arginine in position 74 contributed to an etiologic fraction of 75% in our study population. The PCR-TGGE technique is a simple, nonisotopic method, which may be useful in rapid screening of large populations for HLA disease markers.
- Find related publications in this database (using NLM MeSH Indexing)
- Alleles -
- Autoimmune Diseases - genetics
- Base Sequence - genetics
- DNA Probes, HLA - genetics
- DNA, Single-Stranded - genetics
- Disease Susceptibility - immunology
- Electrophoresis, Polyacrylamide Gel - methods
- Genetic Predisposition to Disease - methods
- Graves Disease - genetics
- HLA-DR Antigens - genetics
- Humans - genetics
- Molecular Sequence Data - genetics
- Nucleic Acid Conformation - genetics
- Polymerase Chain Reaction - genetics
- Risk - genetics
- Temperature - genetics