Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Nauck, M; Winkler, K; März, W; Wieland, H.
Quantitative determination of high-, low-, and very-low-density lipoproteins and lipoprotein(a) by agarose gel electrophoresis and enzymatic cholesterol staining.
Clin Chem. 1995; 41(12 Pt 1):1761-1767
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- Co-Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- Quantification of lipoprotein cholesterol was performed by enzymatic staining of cholesterol in a new agarose gel electrophoresis method that allows the separation of LDL, VLDL, HDL, and lipoprotein(a) [Lp(a)]. Lp(a) shows an electrophoretic mobility clearly distinct from VLDL and HDL. The total CVs of lipoprotein cholesterol varied between 2.7% and 3.9% for LDL, 7.8% and 23.2% for VLDL, 5.2% and 9.5% for HDL, and 6.8% and 16.4% for Lp(a). Comparison of LDL-, VLDL-, and HDL-cholesterol concentrations with the results of a combined ultracentrifugation and precipitation technique gave correlation coefficients of 0.961, 0.947, and 0.918, respectively; comparison of Lp(a)-cholesterol values with those of a nephelometric Lp(a) assay gave r = 0.906. The new electrophoretic assay has several advantages: It allows the quantification of Lp(a)-cholesterol; VLDL-cholesterol is not affected by Lp(a)-cholesterol; and the LDL-cholesterol fraction does not contain Lp(a)-cholesterol, as happens with LDL-cholesterol determined by ultracentrifugation and precipitation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Cholesterol - blood
- Electrophoresis, Agar Gel - blood
- Female - blood
- Humans - blood
- Lipoprotein(a) - blood
- Lipoproteins, HDL - blood
- Lipoproteins, LDL - blood
- Lipoproteins, VLDL - blood
- Male - blood
- Middle Aged - blood
- Staining and Labeling - blood
- Ultracentrifugation - blood
- Find related publications in this database (Keywords)
- ULTRACENTRIFUGATION COMPARED