Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Nauck, M; März, W; Haas, B; Wieland, H.
Homogeneous assay for direct determination of high-density lipoprotein cholesterol evaluated.
Clin Chem. 1996; 42(3):424-429
Doi: 10.1093/clinchem/42.3.424
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- Co-Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- We evaluated a new homogeneous assay for quantifying high-density lipoprotein cholesterol (HDL-C). The assay included four reagents: polyethylene glycol for "wrapping" chylomicrons, very-low-density lipoproteins (VLDL), and low-density lipoproteins (LDL); antibodies specific for apolipoprotein (apo) B and apo C-III to produce aggregates of chylomicrons, VLDL, and LDL; enzymes for the enzymatic cholesterol determination of the noncomplexed lipoproteins with 4-aminoantipyrine as the color reagent; and guanidine salt to stop the enzymatic reaction and to solubilize the complexes of apo B-containing lipoproteins, which would otherwise interfere with the reading of absorbance. The total CVs of the new method ranged between 2.4% and 8.4%. The HDL-C values (y) were in good agreement with those by a comparison phosphotungstic acid/MgCl2 method (x): y= 0.987x + 17.2 mg/L (68th percentile of the residuals on the regression line= 21.49, r= 0.970). At triglyceride concentrations of 20 g/L (Intralipid) the homogeneous HDL-C concentrations increased by 2%. Hemoglobin markedly increased the results, whereas bilirubin reduced them. The homogeneous HDL-C assay was easy to handle and allows full automation. This test should considerably facilitate the screening of individuals at an increased risk of cardiovascular disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoprotein A-I - analysis
- Autoanalysis - analysis
- Cholesterol, HDL - blood
- Chylomicrons - blood
- Evaluation Studies - blood
- Humans - blood
- Indicators and Reagents - blood
- Magnesium Chloride - blood
- Phosphotungstic Acid - blood
- Polyethylene Glycols - blood
- Precipitation - blood
- Quality Control - blood
- Sensitivity and Specificity - blood
- Triglycerides - blood
- Find related publications in this database (Keywords)
- enzyme immunoassay
- apolipoproteins
- screening
- risk factors