Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Cobbaert, C; Zwang, L; Ceriotti, F; Modenese, A; Cremer, P; Herrmann, W; Hoss, G; Jarausch, J; Türk, R; März, W; Nauck, M.
Reference standardization and triglyceride interference of a new homogeneous HDL-cholesterol assay compared with a former chemical precipitation assay.
Clin Chem. 1998; 44(4):779-789
Doi: 10.1093/clinchem/44.4.779
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- Co-Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- A homogeneous HDL-c assay (HDL-H), which uses polyethylene glycol-modified enzymes and sulfated alpha-cyclodextrin, was assessed for precision, accuracy, and cholesterol and triglyceride interference. In addition, its analytical performance was compared with that of a phosphotungstic acid (PTA)/MgCl2 precipitation method (HDL-P). Within-run CVs were < or = 1.87%; total CVs were < or = 3.08%. Accuracy was evaluated in fresh normotriglyceridemic sera using the Designated Comparison Method (HDL-H = 1.037 Designated Comparison Method + 4 mg/L; n = 63) and in moderately hypertriglyceridemic sera by using the Reference Method (HDL-H = 1.068 Reference Method - 17 mg/L; n = 41). Mean biases were 4.5% and 2.2%, respectively. In hypertriglyceridemic sera (n = 85), HDL-H concentrations were increasingly positively biased with increasing triglyceride concentrations. The method comparison between HDL-H and HDL-P yielded the following equation: HDL-H = 1.037 HDL-P + 15 mg/L; n = 478. We conclude that HDL-H amply meets the 1998 NCEP recommendations for total error; its precision is superior compared with that of HDL-P, and its average bias remains below +/-5% as long as triglyceride concentrations are < or = 10 g/L and in case of moderate hypercholesterolemia.
- Find related publications in this database (using NLM MeSH Indexing)
- Cholesterol - blood
- Cholesterol Esterase - blood
- Cholesterol Oxidase - blood
- Cholesterol, HDL - blood
- Cyclodextrins - blood
- Humans - blood
- Hyperlipidemias - blood
- Magnesium Chloride - blood
- Phosphotungstic Acid - blood
- Polyethylene Glycols - blood
- Precipitation - blood
- Reference Standards - blood
- Regression Analysis - blood
- Triglycerides - blood
- Ultracentrifugation - blood