Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Nauck, M; Graziani, MS; Jarausch, J; Bruton, D; Cobbaert, C; Cole, TG; Colella, F; Lefevre, F; Gillery, P; Haas, B; Law, T; König, M; Macke, M; März, W; Meier, C; Riesen, W; van Vliet, M; Wieland, H; Rifai, N.
A new liquid homogeneous assay for HDL cholesterol determination evaluated in seven laboratories in Europe and the United States.
Clin Chem Lab Med. 2000; 37(11-1): 1067-1076.
Doi: 10.1515/CCLM.1999.156
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- We evaluated a new liquid homogeneous assay for the direct measurement of high density lipoprotein cholesterol (HDL-C Plus) in seven laboratories. The assay includes two reagents which can be readily used in most available clinical chemistry analyzers. The total CVs of the new method were below 4.6% and the bias in relation to the designated comparison method was below 3.9%. The total error ranged between 4 to 7%. HDL-C values determined by this method were in good agreement with those obtained by the old homogeneous assay using lyophilized reagents, and other homogeneous and precipitation assays (0.944 < r < 0.996). The assay was linear up to at least 3.89 mmol/l HDL-C. Hemoglobin did not interfere, whereas in icteric samples slight deviations were observed. Lipemia up to 11.3 to 22.6 mmol/l triglycerides did not interfere with this homogeneous HDL-C assay. In samples of patients with paraproteinemia, discrepant results were seen. This liquid homogeneous HDL-C assay was easy to handle and produced similar results in all laboratories participating in this study. This method will enable clinical laboratories to reliably measure HDL-C for risk assessment of coronary heart disease.
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- Artifacts -
- Cholesterol, HDL - blood
- Clinical Chemistry Tests - methods
- Europe - methods
- Humans - methods
- Laboratories - methods
- Reproducibility of Results - methods
- Sensitivity and Specificity - methods
- United States - methods