Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Huber, G; März, W; Martin, JR; Malherbe, P; Richards, JG; Sueoka, N; Ohm, T; Hoffmann, MM.
Characterization of transgenic mice expressing apolipoprotein E4(C112R) and apolipoprotein E4(L28P; C112R).
Neuroscience. 2000; 101(1):211-218
Doi: 10.1016/S0306-4522(00)00341-9
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- Co-Autor*innen der Med Uni Graz
- März Winfried
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- Abstract:
- Apolipoprotein E (ApoE), which is genetically polymorphic, is a constituent of different lipoproteins. Two variants, ApoE4(C112R) and ApoE4(L28P; C112R) have been linked to the risk of developing Alzheimer's disease. Transgenic mice carrying ApoE4(C112R) (AD71) and ApoE4(L28P; C112R) (AD61) were generated and compared to wild-type mice. The use of glial fibrillary acidic protein as promoter led to transgene expression mainly in glial cells but also in neurons. Transgene protein levels were approximately three-and-a-half-fold that of endogenous ApoE in the glial fibrillary acidic protein-ApoE4(C112R) (AD71) and nearly twofold in the glial fibrillary acidic protein-ApoE4(L28P; C112R) (AD61) mouse lines. Neither transgenic mouse differed from wild-type in cognitive tests at the age of approximately one-and-a-half years. The locomotor activity of AD61 mice was similar to controls, whereas AD71 mice exhibited a clearly reduced level of motor activity. Immunohistological and biochemical brain protein analyses revealed no difference between strains.Thus, in the absence of morphological changes over-expression of ApoE4(C112R) on a background of endogenous mouse ApoE, may result in behavioral deficits while for the ApoE4(L28P; C112R) transgene higher expression might be required or some compensatory mechanisms might protect these animals from the behavioral abnormalities.
- Find related publications in this database (using NLM MeSH Indexing)
- Alzheimer Disease - genetics
- Animals -
- Apolipoprotein E4 -
- Apolipoproteins E - genetics
- Brain - metabolism
- Gene Expression Regulation - physiology
- Mice -
- Mice, Transgenic - genetics
- Motor Activity - genetics
- Nerve Tissue Proteins - metabolism
- Neuroglia - metabolism
- Neurons - metabolism
- Phenotype -
- Protein Isoforms - genetics
- Risk Factors -
- Transgenes - genetics
- Find related publications in this database (Keywords)
- ApoE
- behavior
- motor activity
- transgenic mice