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SHR Neuro Cancer Cardio Lipid Metab Microb
Walter, J; Joffe, B; Bolzer, A; Albiez, H; Benedetti, PA; Müller, S; Speicher, MR; Cremer, T; Cremer, M; Solovei, I.
Towards many colors in FISH on 3D-preserved interphase nuclei.
Cytogenet Genome Res. 2006; 114(3-4): 367-378.
Doi: 10.1159/000094227
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- Co-authors Med Uni Graz
- Speicher Michael
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- Abstract:
- The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome - one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Nucleus - genetics
- Chromosome Painting - genetics
- Color - genetics
- Humans - genetics
- In Situ Hybridization, Fluorescence - methods
- Interphase - genetics
- Microscopy, Fluorescence - genetics