Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koidl, C; Bozic, M; Burmeister, A; Hess, M; Marth, E; Kessler, HH.
Detection and differentiation of Bordetella spp. by real-time PCR.
J Clin Microbiol. 2007; 45(2): 347-350.
Doi: 10.1128/JCM.01303-06
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- Führende Autor*innen der Med Uni Graz
- Kessler Harald
- Koidl Christoph
- Co-Autor*innen der Med Uni Graz
- Bozic Michael
- Marth Egon
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- Abstract:
- Molecular detection of Bordetella pertussis DNA is a sensitive and specific method for the rapid diagnosis of pertussis. In this study, a new molecular assay for the detection and differentiation of Bordetella spp. based on automated DNA extraction and real-time PCR was evaluated. The analytical sensitivity of the new assay was determined by Probit analysis of serial dilutions of both cloned PCR products IS481 and IS1001 and cell suspensions of B. pertussis, B. parapertussis, and B. bronchiseptica. The specificity was analyzed by testing a number of pathogens producing respiratory infections. Moreover, a total of 92 clinical samples were investigated. The results were compared to those obtained by an in-house assay based on manual DNA extraction, followed by real-time PCR and detection of IS481. The analytical sensitivity of the new assay for the detection of IS481 and IS1001 was determined to be 2.2 and 1.2 genome equivalents/mul, respectively. The analytical sensitivity for the detection of B. pertussis, B. parapertussis, and B. bronchiseptica was determined to be 1.6, 1.0, and 2.7 genome equivalents/mul, respectively. When clinical specimens were tested with the new assay, 46 of 92 were found to be positive for Bordetella DNA. With the in-house assay, 45 samples tested positive. The new molecular assay proved to be suitable for the rapid diagnosis of pertussis in the routine diagnostic laboratory.
- Find related publications in this database (using NLM MeSH Indexing)
- Bordetella - classification
- Bordetella Infections - diagnosis
- Bordetella bronchiseptica - classification
- Bordetella parapertussis - classification
- Bordetella pertussis - classification
- DNA, Bacterial - analysis
- Humans - analysis
- Polymerase Chain Reaction - methods
- Sensitivity and Specificity - methods
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