Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Anaid Shahbazian; Petkov, V; Baykuscheva-Gentscheva, T; Hoeger, H; Painsipp, E; Holzer, P; Mosgoeller, W.
Involvement of endothelial NO in the dilator effect of VIP on rat isolated pulmonary artery.
Regul Pept. 2007; 139(1-3): 102-108.
Doi: 10.1016/j.regpep.2006.10.012
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- Co-Autor*innen der Med Uni Graz
- Holzer Peter
- Singewald Evelin
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- Abstract:
- The endothelium and its interaction with smooth muscle play a central role in the local control of the pulmonary vasculature, and endothelial dysfunction is thought to contribute to pulmonary hypertension and chronic obstructive pulmonary disease. Vasoactive intestinal peptide (VIP), a 28-amino acid neuropeptide, relaxes the rat pulmonary artery, but there is controversy as to whether or not this action of VIP depends on the endothelium. The aim of this study, therefore, was to investigate the role of the endothelium and nitric oxide (NO), the major endothelium-derived relaxing factor, in the dilator action of VIP on the rat isolated pulmonary artery. Pulmonary artery preparations pre-contracted by the alpha(1)-adrenoceptor agonist L-phenylephrine were relaxed by VIP (0.003-1 microM) and acetylcholine (0.003-10 microM) in a concentration-dependent manner. Mechanical removal of the endothelium reduced the maximal response to VIP by about 50% and practically abolished the response to acetylcholine. Inhibition of NO synthesis by N(omega)-nitro-L-arginine methyl ester (0.5 mM) had a similar effect, abolishing the vasorelaxation caused by acetylcholine and attenuating the vasorelaxation caused by VIP by about 50%. From these data it is concluded that the relaxant action of VIP on the rat isolated pulmonary artery depends in part on the presence of the endothelium and that this part is mediated by endothelial NO.
- Find related publications in this database (using NLM MeSH Indexing)
- Acetylcholine - pharmacology
- Animals - pharmacology
- Endothelium, Vascular - drug effects
- Male - drug effects
- Muscle Relaxation - drug effects
- Muscle, Smooth, Vascular - drug effects
- NG-Nitroarginine Methyl Ester - pharmacology
- Nitric Oxide - metabolism
- Nitric Oxide Synthase - antagonists and inhibitors
- Pulmonary Artery - drug effects
- Rats - drug effects
- Rats, Sprague-Dawley - drug effects
- Vasoactive Intestinal Peptide - pharmacology
- Vasodilation - drug effects
- Find related publications in this database (Keywords)
- vasoactive intestinal peptide
- acetylcholine
- nitric oxide
- N omega-nitro-L-arginine methyl ester
- vascular endothelium
- rat pulmonary artery
- endothelium-dependent relaxation