Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Büttner, S; Eisenberg, T; Carmona-Gutierrez, D; Ruli, D; Knauer, H; Ruckenstuhl, C; Sigrist, C; Wissing, S; Kollroser, M; Fröhlich, KU; Sigrist, S; Madeo, F.
Endonuclease G regulates budding yeast life and death.
Mol Cell. 2007; 25(2):233-246
Doi: 10.1016/j.molcel.2006.12.021
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- Co-Autor*innen der Med Uni Graz
- Kollroser Manfred
- Ruckenstuhl Rudolf
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- Abstract:
- Endonuclease G (EndoG) is located in mitochondria yet translocates into the nucleus of apoptotic cells during human degenerative diseases. Nonetheless, a direct involvement of EndoG in cell-death execution remains equivocal, and the mechanism for mitochondrio-nuclear translocation is not known. Here, we show that the yeast homolog of EndoG (Nuc1p) can efficiently trigger apoptotic cell death when excluded from mitochondria. Nuc1p induces apoptosis in yeast independently of metacaspase or of apoptosis inducing factor. Instead, the permeability transition pore, karyopherin Kap123p, and histone H2B interact with Nuc1p and are required for cell death upon Nuc1p overexpression, suggesting a pathway in which mitochondrial pore opening, nuclear import, and chromatin association are successively involved in EndoG-mediated death. Deletion of NUC1 diminishes apoptotic death when mitochondrial respiration is increased but enhances necrotic death when oxidative phosphorylation is repressed, pointing to dual--lethal and vital--roles for EndoG.
- Find related publications in this database (using NLM MeSH Indexing)
- Active Transport, Cell Nucleus -
- Amino Acid Sequence -
- Apoptosis - physiology
- Carrier Proteins - genetics Carrier Proteins - metabolism
- Caspases - genetics Caspases - metabolism
- Endodeoxyribonucleases - genetics Endodeoxyribonucleases - metabolism
- Endonucleases - genetics Endonucleases - metabolism
- Gene Deletion -
- Gene Expression -
- Genes, Fungal -
- Histones - metabolism
- Mitochondria - enzymology
- Models, Biological -
- Molecular Sequence Data -
- NADH, NADPH Oxidoreductases - genetics NADH, NADPH Oxidoreductases - metabolism
- Phosphorylation -
- Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics
- Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism
- Sequence Homology, Amino Acid -
- beta Karyopherins -