Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Ligr, M; Madeo, F; Fröhlich, E; Hilt, W; Fröhlich, KU; Wolf, DH.
Mammalian Bax triggers apoptotic changes in yeast.
FEBS Lett. 1998; 438(1-2):61-65
Doi: 10.1016/S0014-5793(98)01227-7
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- Co-Autor*innen der Med Uni Graz
- Fröhlich Eleonore
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- Abstract:
- Apoptosis is co-regulated by the conserved family of Bcl-2-related proteins, which includes both its agonists (Bax) and antagonists (Bcl-X(L)). A mutant strain of the yeast Saccharomyces cerevisiae has been shown to express all morphological signs of apoptosis. Overexpression of Bax is lethal in S. cerevisiae, whereas simultaneous overexpression of Bcl-X(L) rescues the cells. We report that overexpression of mammalian Bax in a S. cerevisiae wild type strain triggers morphological changes similar to those of apoptotic metazoan cells: the loss of asymmetric distribution of plasma membrane phosphatidylserine, plasma membrane blebbing, chromatin condensation and margination, and DNA fragmentation. Simultaneous overexpression of Bcl-X(L) prevents these changes. We demonstrate that Bax triggers phenotypic alterations in yeast strongly resembling those it causes in metazoan apoptotic cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis -
- Cell Membrane - metabolism
- Cell Nucleus - metabolism
- Chromatin - metabolism
- DNA Fragmentation - metabolism
- Gene Transfer Techniques - metabolism
- In Situ Nick-End Labeling - metabolism
- Indoles - metabolism
- Mammals - genetics
- Microscopy, Electron - genetics
- Phosphatidylserines - metabolism
- Proto-Oncogene Proteins - genetics
- Proto-Oncogene Proteins c-bcl-2 - genetics
- Saccharomyces cerevisiae - cytology
- bcl-2-Associated X Protein - cytology
- bcl-X Protein - cytology
- Find related publications in this database (Keywords)
- apoptosis
- Bax
- yeast Saccharomyces cerevisiae