Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fröhlich, E; Witke, A; Czarnocka, B; Wahl, R.
Retinol has specific effects on binding of thyrotrophin to cultured porcine thyrocytes.
J Endocrinol. 2004; 183(3):617-626
Doi: 10.1677/joe.1.05693
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- Führende Autor*innen der Med Uni Graz
- Fröhlich Eleonore
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- Abstract:
- Retinoids are potential candidates for the treatment of thyroid cancer. However, one of the disadvantages of these substances is their dedifferentiating effect on normal non-transformed thyrocytes. To identify conditions under which no dedifferentiating effect of retinol on normal thyrocytes can be observed, we determined iodide uptake, protein iodination, expression of sodium-iodide symporter (NIS) mRNA and protein, and the binding of iodine-125-labelled bTSH in cultured porcine thyrocytes. Combination of TSH and < or =6.5 microM retinol increased iodide uptake and protein iodination compared with TSH alone over the entire incubation time, whereas TSH plus > or =13 microM retinol increased the uptake of iodine-125 only during the first 12 h but decreased it after 30 h and longer. After > or =30 h incubation times with > or =13 microM retinol, the fraction of apoptotic cells was enhanced and proliferation decreased. The incubation with retinol enhanced the binding of [125I]bTSH to thyrocytes, but did not influence expression of the NIS. With low retinol concentrations, the effect on the binding of TSH apparently predominated and retinol increased thyroid function; with higher concentrations the pro-apoptotic effect of retinol overlapped and a two-phased time course resulted. It can be concluded that low concentrations of retinol also exert differentiating effects in normal thyrocytes.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis - drug effects
- Cell Proliferation - drug effects
- Cells, Cultured - drug effects
- Immunohistochemistry - methods
- Iodides - metabolism
- Iodine Radioisotopes - metabolism
- Protein Binding - drug effects
- RNA, Messenger - analysis
- Swine - analysis
- Symporters - genetics
- Thyroid Gland - cytology
- Thyrotropin - metabolism
- Time Factors - metabolism
- Vitamin A - pharmacology