Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Fröhlich, E; Maier, E; Klessen, C.
Isolation of bovine retinal pigment epithelial cells using adhesion to agarose: demonstration of cellular and regional heterogeneity.
J Histochem Cytochem. 2003; 51(1):121-124
Doi: 10.1177/002215540305100114
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- Führende Autor*innen der Med Uni Graz
- Fröhlich Eleonore
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- Abstract:
- The retinal pigment epithelium (RPE) shows cell heterogeneity in morphology and enzymatic activity. Routine isolation procedures for RPE cells may reduce enzymatic activity and prevent the quantification of regional enzymatic differences in vivo. We developed a new technique for the isolation of RPE cells based on adhesion of the cells to agarose. The morphology of the isolated cells resembled that of RPE cells in vivo. The cells were viable in the dye exclusion test and showed a histochemical staining pattern as RPE cells in vivo. With this technique, quantitative regional differences in the enzymatic activities were detected.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cattle -
- Cell Adhesion -
- Female -
- Pigment Epithelium of Eye - cytology
- Retina - cytology
- Sepharose - cytology
- Find related publications in this database (Keywords)
- retinal pigment epithelium
- ultrastructure
- enzymatic activity