Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Biedermann, B; Fröhlich, E; Grosche, J; Wagner, HJ; Reichenbach, A.
Mammalian Müller (glial) cells express functional D2 dopamine receptors.
Neuroreport. 1995; 6(4): 609-612.
Doi: 10.1097/00001756-199503000-00006
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PubMed
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- Co-Autor*innen der Med Uni Graz
- Fröhlich Eleonore
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- Abstract:
- Dopamine plays important functional roles in the vertebrate retina. Here we show that functional D2 dopamine receptors are present on mammalian retinal Müller (glial) cells. Using an antiserum directed to two oligopeptides predicted from rat D2 receptor DNA, patchy label was demonstrated immunocytochemically on virtually all Müller cells enzymatically isolated from guinea-pig and rat retinae. Application of exogeneous dopamine to voltage-clamped isolated living guinea-pig Müller cells caused either a decrease (40%), an increase (32%), or no change (28%) of the input resistance of the membrane. The D2 receptor agonist quinelorane caused an increase of the membrane's input resistance in 100% of the cells. This effect was completely blocked by the D2 receptor antagonist S(-)-sulpiride. When all voltage-activated K+ channels except the delayed rectifiers were blocked by Ba2+, quinelorane had no effect. Further, the reversal potentials of the responses were near the potassium equilibrium potential. We conclude that the activation of Müller cell D2 receptors closes (inwardly rectifying) K+ channels. The presence of functional dopamine receptors on mammalian Müller cells may have important consequences for retinal K+ clearance, and thus, for information processing in the retina.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Guinea Pigs -
- Immunohistochemistry -
- Membrane Potentials - physiology
- Neuroglia - chemistry
- Rats - chemistry
- Receptors, Dopamine D2 - analysis
- Retina - chemistry
- Find related publications in this database (Keywords)
- RETINA
- GLIA
- CELL ISOLATION
- IMMUNOCYTOCHEMISTRY
- ELECTROPHYSIOLOGY
- PATCH CLAMP
- DOPAMINE
- RECEPTORS