Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Groschner, K; Graier, WF; Kukovetz, WR.
Activation of a small-conductance Ca(2+)-dependent K+ channel contributes to bradykinin-induced stimulation of nitric oxide synthesis in pig aortic endothelial cells.
Biochim Biophys Acta. 1992; 1137(2):162-170
Doi: 10.1016/0167-4889(92)90198-K
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Groschner Klaus
- Co-Autor*innen der Med Uni Graz
- Graier Wolfgang
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Bradykinin-induced K+ currents, membrane hyperpolarization, as well as rises in cytoplasmic Ca2+ and cGMP levels were studied in endothelial cells cultured from pig aorta. Exposure of endothelial cells to 1 microM bradykinin induced a whole-cell K+ current and activated a small-conductance (approximately 9 pS) K+ channel in on-cell patches. This K+ channel lacked voltage sensitivity, was activated by increasing the Ca2+ concentration at the cytoplasmic face of inside-out patches and blocked by extracellular tetrabutylammonium (TBA). Bradykinin concomitantly increased membrane potential and cytoplasmic Ca2+ of endothelial cells. In high (140 mM) extracellular K+ solution, as well as in the presence of the K(+)-channel blocker TBA (10 mM), bradykinin-induced membrane hyperpolarization was abolished and increases in cytoplasmic Ca2+ were reduced to a slight transient response. Bradykinin-induced rises in intracellular cGMP levels which reflect Ca(2+)-dependent formation of EDRF(NO) were clearly attenuated in the presence of TBA (10 mM). Our results suggest that bradykinin hyperpolarizes pig aortic endothelial cells by activation of small-conductance Ca(2+)-activated K+ channels. Opening of these K+ channels results in membrane hyperpolarization which promotes Ca2+ entry, and consequently, NO synthesis.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bradykinin - antagonists and inhibitors Bradykinin - pharmacology
- Calcium - metabolism
- Cells, Cultured -
- Cyclic GMP - metabolism
- Cytoplasm - metabolism
- Detergents - pharmacology
- Endothelium, Vascular - cytology Endothelium, Vascular - metabolism
- Membrane Potentials -
- Nitric Oxide - metabolism
- Potassium Channels - metabolism
- Quaternary Ammonium Compounds - pharmacology
- Swine -
- Find related publications in this database (Keywords)
- ENDOTHELIAL CELL
- POTASSIUM CHANNEL, CALCIUM ION ACTIVATED
- CALCIUM ION, INTRACELLULAR
- EDRF/NO
- (PIG AORTA)