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Domiati-Saad, R; Attrep, JF; Brezinschek, HP; Cherrie, AH; Karp, DR; Lipsky, PE.
Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis.
J Immunol. 1996; 156(10):3608-3620 [OPEN ACCESS]
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Co-authors Med Uni Graz: Brezinsek Hans-Peter
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Abstract:: Staphylococcal enterotoxins are potent superantigens, in that they activate T cells bearing specific V beta-chain gene segments. In this study, we analyzed the capacity of staphylococcal enterotoxin D (SED) to function as a B cell superantigen. SED induced T cell-dependent polyclonal proliferation and differentiation of B cells. In the absence of T cells, SED induced survival of B cells uniquely expressing VH4 containing IgM. The mechanism of survival of VH4-expressing B cells appeared to relate to the countering of apoptosis initiated by the engagement of HLA-DR by SED. Analysis of the VH4 gene products expressed by SED-stimulated B cells revealed the usage of six of the known functional VH4 genes with a variety of different CDR3 regions, employing different DH and JH gene segments. Moreover, the sequence analysis identified a possible site for SED binding of VH4 that includes the solvent-exposed surfaces of 3' CDR2/FR3 and/or FR1. Thus, SED appears to function as a unique B cell superantigen by inducing survival of VH4-expressing B cells.
Find related publications in this database (using NLM MeSH Indexing): Adult -; Amino Acid Sequence -; Antibody Diversity -; Apoptosis - drug effects; B-Lymphocytes - chemistry; Base Sequence - chemistry; Cell Division - drug effects; Enterotoxins - pharmacology; Genes, Immunoglobulin - drug effects; Humans - drug effects; Immunoglobulin Heavy Chains - chemistry; Immunoglobulin Variable Region - chemistry; Lymphocyte Activation - drug effects; Molecular Sequence Data - drug effects; Recombinant Proteins - pharmacology; Superantigens - pharmacology; T-Lymphocytes - immunology