Medizinische Universität Graz - Research portal
Selected Publication:
Brezinschek, HP; Brezinschek, RI; Dörner, T; Lipsky, PE.
Similar characteristics of the CDR3 of V(H)1-69/DP-10 rearrangements in normal human peripheral blood and chronic lymphocytic leukaemia B cells.
Br J Haematol. 1998; 102(2):516-521
Doi: 10.1046/j.1365-2141.1998.00787.x
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- Leading authors Med Uni Graz
- Brezinsek Hans-Peter
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- Abstract:
- The variable heavy chain (V(H)) gene segment V(H)1-69/DP-10 has been shown to be over-represented in B-cell chronic lymphocytic leukaemia (CLL). Because of certain similar characteristics of their complementarity determining region 3 (CDR3), including preferential utilization of J(H)6 elements and an extended length, it has been suggested that antigenic stimulation might be involved in leukaemogenesis. Utilizing single-cell PCR to amplify and sequence genomic DNA from individual normal human peripheral blood B cells, we have obtained 7/421 productively and 1/69 nonproductively rearranged V(H) genes that used V(H)1-69/DP-10. All productive rearrangements were unmutated, used J(H)6 and had an average CDR3 length similar to that previously found in V(H)1-69/DP-10-expressing CLL cells. These results suggest that CLL may arise from B cells commonly found in the peripheral B-cell repertoire and do not represent expansion of a unique subset of specific antigen-reactive B cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- B-Lymphocytes - chemistry
- Female - chemistry
- Gene Rearrangement, B-Lymphocyte, Heavy Chain - genetics
- Humans - genetics
- Leukemia, B-Cell - blood
- Male - blood
- Polymerase Chain Reaction - methods
- Sequence Analysis, DNA - methods
- Find related publications in this database (Keywords)
- CDR3
- chronic lymphocytic leukaemia
- Ig rearrangements
- leukaemogenesis
- single-cell PCR