Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Guelly, C; Küpcü, Z; Zalusky, D; Karner, M; Zehetner, M; Schweighoffer, T.
Activation requirements of circulating antigen-specific human CD8(+) memory T cells probed with insect cell-based artificial antigen-presenting cells.
Eur J Immunol. 2002; 32(1):182-192
Doi: 10.1002/1521-4141(200201)32:1<182::AID-IMMU182>3.3.CO;2-G
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- Führende Autor*innen der Med Uni Graz
- Gülly Christian
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- Abstract:
- We sought to define the molecular setup of an antigen-presenting cell that elicits antigen-specific T cell responses in vitro using insect cells that were infected with recombinant baculoviruses. Expression of single-chain HLA was complemented step-by-step with costimulatory molecules, including CD54 and CD80, by co-infection with the relevant viruses. Role of CD8 was assessed by introducing hybrid class I molecules where the alpha-3 domain of the HLA heavy chain molecule was replaced by its murine K(b) counterpart. Circulating T cells that respond to the EBV-derived HLA-A2-restricted peptide GLGCTLVAML were previously shown to bear hallmarks of memory cells. We found that the HLA+peptide complex alone displayed on the surface of insect cells was sufficient to elicit IFN-gamma secretion from these freshly isolated CD8(+) T cells in ELISpot assays. Binding of CD8 was absolutely required, but coexpression of costimulatory molecules resulted only in minimal increase in the number of spots. Tumor antigen-specific CTL clones also reacted in a strictly antigen-specific manner, but required CD54 for quantitative responses. The amount of IFN-gamma produced by the individual reactive T cells was evaluated as spot size, and was also influenced by the costimulatory molecules: CD54 increased also the response magnitude of cultured CTL lines, while CD80 enhanced cytokine release from freshly isolated CD8(+) T cells. Understanding the stimulatory requirements of functionally competent effector/memory T cells and their exact enumeration will be helpful for increasing the efficacy of vaccines.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigen-Presenting Cells - immunology
- Antigens, CD80 - genetics
- Antigens, Neoplasm - immunology
- Antigens, Viral - immunology
- Baculoviridae - immunology
- CD8-Positive T-Lymphocytes - immunology
- Cell Line - immunology
- Gene Expression - immunology
- Genetic Vectors - immunology
- HLA-A2 Antigen - genetics
- Health Status - genetics
- Humans - genetics
- Immunoglobulin Fragments - genetics
- Immunoglobulin Heavy Chains - genetics
- Immunologic Memory - immunology
- Insects - immunology
- Intercellular Adhesion Molecule-1 - genetics
- Leukocytes, Mononuclear - immunology
- Lymphocyte Activation - immunology
- Mice - immunology
- Peptides - immunology
- Protein Binding - immunology
- Recombinant Fusion Proteins - genetics
- Spodoptera - cytology
- beta 2-Microglobulin - genetics
- Find related publications in this database (Keywords)
- memory
- MHC
- T lymphocyte
- costimulatory molecule