Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gruber, R; Graninger, W; Bobacz, K; Watzek, G; Erlacher, L.
BMP-6-induced osteogenic differentiation of mesenchymal cell lines is not modulated by sex steroids and resveratrol.
Cytokine. 2003; 23(4-5):133-137
Doi: 10.1016/S1043-4666(03)00223-0
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- Co-Autor*innen der Med Uni Graz
- Graninger Winfried
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- Abstract:
- Bone morphogenetic protein-6 (BMP-6) is a potent inducer of osteogenic differentiation and its expression is stimulated by 17beta-estradiol. The existence of a regulatory loop between sex steroids and BMP-6 is therefore reasonable to hypothesize. Here we determined whether the sex steroids 17beta-estradiol and dihydrotestosterone, and the phytoestrogen resveratrol can modulate BMP-6-induced alkaline phosphatase activity and osteocalcin expression. Mesenchymal cells of murine (osteoblastic MC3T3-E1 cells, preadipogenic ST2 cells, prechondrogenic ATDC5 cell) and human origin (osteosarcoma SaOS and HOS cells, primary bone marrow stromal cells) were cultured in the presence of recombinant BMP-6 under serum-free conditions. BMP-6 dose-, and time-dependently increased alkaline phosphatase activity in murine cell lines, but not in human cells. Osteocalcin expression was also increased upon stimulation with BMP-6. The presence of 17beta-estradiol, dihydrotestosterone, and resveratrol had no effect on BMP-6-induced alkaline phosphatase activity and osteocalcin expression. These data suggest that osteogenic differentiation in response to BMP-6 occurs independent of steroid hormones and resveratrol in mesenchymal cells that express basal receptor levels.
- Find related publications in this database (using NLM MeSH Indexing)
- Alkaline Phosphatase - metabolism
- Animals - metabolism
- Blotting, Northern - metabolism
- Bone Morphogenetic Proteins - pharmacology
- Cell Differentiation - drug effects
- Cell Line - drug effects
- Cell Line, Tumor - drug effects
- Dihydrotestosterone - pharmacology
- Dose-Response Relationship, Drug - pharmacology
- Estradiol - pharmacology
- Gene Expression Regulation - drug effects
- Gonadal Steroid Hormones - pharmacology
- Humans - pharmacology
- Mesenchymal Stem Cells - drug effects
- Mesoderm - cytology
- Mice - cytology
- Osteocalcin - genetics
- Osteogenesis - drug effects
- RNA, Messenger - drug effects
- Reverse Transcriptase Polymerase Chain Reaction - drug effects
- Stilbenes - pharmacology
- Time Factors - pharmacology