Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gruber, R; Mayer, C; Schulz, W; Graninger, W; Peterlik, M; Watzek, G; Luyten, FP; Erlacher, L.
Stimulatory effects of cartilage-derived morphogenetic proteins 1 and 2 on osteogenic differentiation of bone marrow stromal cells.
Cytokine. 2000; 12(11):1630-1638
Doi: 10.1006/cyto.2000.0760
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Graninger Winfried
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- Abstract:
- Cartilage-derived morphogenetic proteins 1 and 2 (CDMP-1 and CDMP-2) are members of the bone morphogenetic protein (BMP) family which play an important role in embryonic skeletal development. Throughout adult life, bone marrow-derived precursor cells maintain their ability to differentiate into osteoblasts in response to local growth factors. This study examines the osteogenic potential of CDMP-1, CDMP-2, BMP-6 and osteogenic protein 1 (OP-1) in bone marrow stromal cells (BMSC) and investigates the endogenous expression of CDMPs/BMPs and their respective activin receptor-like kinase (ALK) receptors. A 4-day exposure of BMSC to CDMP-1, CDMP-2, BMP-6, and OP-1 under serum-free conditions stimulated the progression of the osteogenic lineage in a dose-dependent manner as evaluated by alkaline phosphatase activity and osteocalcin synthesis. In contrast to the BMPs, CDMP-1 and especially CDMP-2 were significantly less osteogenic, as confirmed by Northern blot analysis. Moreover, BMSC were shown to express endogenously CDMP-2, BMP-2 to -6 and ALK-1, -2, -3, -5 and -6. Phenotypic characterization of BMSC by RT-PCR showed transcripts of the fat marker adipsin and the prechondrocytic marker procollagen type IIA; however, we were unable to detect the mature cartilage markers, procollagen type IIB and aggrecan, even after growth factor treatment. Our data indicate that CDMP-1, CDMP-2, BMP-6 and OP-1 enhance the osteogenic phenotype in BMSC, with CDMPs being clearly less osteogenic than BMPs. The endogenous expression of a variety of CDMPs/BMPs and their respective ALK receptors, suggests a possible involvement of these growth factors in the osteogenic differentiation of bone marrow progenitor cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Activin Receptors -
- Alkaline Phosphatase - metabolism
- Animals - metabolism
- Blotting, Northern - metabolism
- Bone Marrow Cells - metabolism
- Bone Morphogenetic Proteins - metabolism
- Bone and Bones - cytology
- Cell Differentiation - cytology
- Cell Division - drug effects
- Complement Factor D - drug effects
- Culture Media, Serum-Free - metabolism
- DNA Primers - metabolism
- Dose-Response Relationship, Drug - metabolism
- Growth Substances - metabolism
- Humans - metabolism
- Mice - metabolism
- Osteocalcin - metabolism
- Phenotype - metabolism
- Procollagen - metabolism
- Protein-Serine-Threonine Kinases - metabolism
- Reverse Transcriptase Polymerase Chain Reaction - metabolism
- Serine Endopeptidases - metabolism
- Stromal Cells - metabolism
- Temperature - metabolism
- Transforming Growth Factor beta - metabolism
- Find related publications in this database (Keywords)
- ALK-receptors
- bone marrow stromal cells
- bone morphogenetic proteins
- cartilage-derived morphogenetic proteins
- osteogenic differentiation