Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Scalera, F; Fischer, T; Schlembach, D; Beinder, E.
Serum from healthy pregnant women reduces oxidative stress in human umbilical vein endothelial cells.
Clin Sci (Lond). 2002; 103(1):53-57
Doi: 10.1042/cs1030053
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- Co-Autor*innen der Med Uni Graz
- Schlembach Dietmar
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- Abstract:
- This study was conducted to compare the effects of serum from healthy pregnant women and that from pregnant women with pre-eclampsia on oxidative stress in endothelial cells in culture. Human umbilical vein endothelial cells (HUVECs) were incubated with serum from 18 pre-eclamptic, 18 healthy pregnant and 18 healthy non-pregnant women for 24 h. The levels of reduced glutathione (GSH) and lipid peroxides (LPOs) were measured in endothelial cell lysates. Measurement of malondialdehyde in combination with 4-hydroxyalkenals has been used as an indicator of LPOs. Serum from healthy pregnant women decreased significantly the LPO content in HUVECs in comparison with serum from pre-eclamptic women and healthy non-pregnant women (30.7+/-6.6 compared with 39.3+/-10.9 and 41.0+/-12.7 pmol/mg of protein respectively; P<0.003 and P<0.01 respectively). No differences in GSH content between the three groups (18.3+/-2.1 nmol/mg of protein for healthy pregnant, 19.2+/-3.3 nmol/mg for pre-eclamptic and 18.3+/-2.0 nmol/mg for healthy non-pregnant women) were found. Thus serum from normal pregnant women contains a factor(s) that decreases oxidative stress in human endothelial cells. This mechanism might be altered in pre-eclampsia.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Cells, Cultured -
- Endothelium, Vascular - cytology
- Female - cytology
- Glutathione - biosynthesis
- Humans - biosynthesis
- Lipid Peroxidation - biosynthesis
- Lipid Peroxides - biosynthesis
- Oxidative Stress - biosynthesis
- Pre-Eclampsia - blood
- Pregnancy - blood
- Umbilical Veins - cytology
- Find related publications in this database (Keywords)
- glutathione
- human umbilical vein endothelial cells
- lipid peroxides
- pre-eclampsia
- serum