Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Scalera, F; Schlembach, D; Beinder, E.
Production of vasoactive substances by human umbilical vein endothelial cells after incubation with serum from preeclamptic patients.
Eur J Obstet Gynecol Reprod Biol. 2001; 99(2):172-178
Doi: 10.1016/S0301-2115(01)00412-2
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- Co-Autor*innen der Med Uni Graz
- Schlembach Dietmar
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- Abstract:
- OBJECTIVE: To determine the in vitro effect of serum from preeclamptic patients on the proliferation, viability and secretion of vasoactive substances by human umbilical vein endothelial cells (HUVEC). STUDY DESIGN: HUVEC were incubated for 24h with sera from 16 preeclamptic, 19 healthy pregnant and 8 healthy nonpregnant women. Proliferation rates were determined by cell counting and vitality by trypan blue staining. The vasoactive substances, 6-keto-prostaglandin F(1alpha), nitrite and nitrate, and endothelin-1 (ET-1) were measured in endothelial cell supernatants. RESULTS: The preeclamptic serum had no effect on cell proliferation or vitality compared with control sera. It induced more HUVEC production of ET-1, but not of prostacyclin (PGI2) or nitric oxide compared with control serum. CONCLUSION: Preeclamptic serum appears to contain a factor(s) that specifically stimulates ET-1 secretion from HUVEC without altering cell growth or vitality.
- Find related publications in this database (using NLM MeSH Indexing)
- 6-Ketoprostaglandin F1 alpha - biosynthesis
- Adult - biosynthesis
- Blood - biosynthesis
- Cell Division - biosynthesis
- Cells, Cultured - biosynthesis
- Culture Media - biosynthesis
- Endothelin-1 - biosynthesis
- Endothelium, Vascular - metabolism
- Female - metabolism
- Humans - metabolism
- Nitrates - metabolism
- Nitric Oxide - biosynthesis
- Nitrites - metabolism
- Pre-Eclampsia - blood
- Pregnancy - blood
- Umbilical Veins - blood
- Find related publications in this database (Keywords)
- preeclampsia
- human umbilical vein endothelial cells
- endothelial dysfunction
- vasoactive substances