Medizinische Universität Graz - Research portal
Selected Publication:
Marschitz, I; Lechner, J; Mosser, I; Dander, M; Montesano, R; Schramek, H.
Differential expression of cell-cell adhesion proteins and cyclin D in MEK1-transdifferentiated MDCK cells.
Am J Physiol Cell Physiol. 2000; 279(5):C1472-C1482
Doi: 10.1152/ajpcell.2000.279.5.C1472
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Marschitz Ingrid Christine
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Overexpression of a constitutively active mutant of the mitogen-activated protein kinase kinase MEK1 (caMEK1) in epithelial Madin-Darby canine kidney (MDCK)-C7 cells disrupts morphogenesis, induces an invasive phenotype, and is associated with a reduced rate of cell proliferation. The role of cell-cell adhesion molecules and cell cycle proteins in these processes, however, has not been investigated. We now report loss of E-cadherin expression as well as a marked reduction of beta- and alpha-catenin expression in transdifferentiated MDCK-C7 cells stably expressing caMEK1 (C7caMEK1) compared with epithelial mock-transfected MDCK-C7 (C7Mock1) cells. At least part of the remaining alpha-catenin was coimmunoprecipitated with beta-catenin, whereas no E-cadherin was detected in beta-catenin immunoprecipitates. In both cell types, the proteasome-specific protease inhibitors N-acetyl-Leu-Leu-norleucinal (ALLN) and lactacystin led to a time-dependent accumulation of beta-catenin, including the appearance of high-molecular-weight beta-catenin species. Quiescent as well as serum-stimulated C7caMEK1 cells showed a higher cyclin D expression than epithelial C7Mock1 cells. The MEK inhibitor U-0126 inhibited extracellular signal-regulated kinase phosphorylation and cyclin D expression in C7caMEK1 cells and almost abolished their already reduced cell proliferation rate. We conclude that the transdifferentiated and invasive phenotype of C7caMEK1 cells is associated with a diminished expression of proteins involved in cell-cell adhesion. Although beta-catenin expression is reduced, C7caMEK1 cells show a higher expression of U-0126-sensitive cyclin D protein.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cadherins - metabolism
- Cell Adhesion Molecules - metabolism
- Cell Differentiation - physiology
- Cell Division - physiology
- Cell Line - cytology
- Cyclins - metabolism
- Cysteine Endopeptidases - physiology
- Cytoskeletal Proteins - metabolism
- Dogs - metabolism
- Kidney - cytology
- MAP Kinase Kinase 1 - cytology
- Mitogen-Activated Protein Kinase Kinases - genetics
- Multienzyme Complexes - physiology
- Peptide Hydrolases - metabolism
- Proteasome Endopeptidase Complex - metabolism
- Protein-Serine-Threonine Kinases - genetics
- Research Support, Non-U.S. Gov't - genetics
- Trans-Activators - genetics
- Transfection - genetics
- alpha Catenin - genetics
- beta Catenin - genetics
- Find related publications in this database (Keywords)
- extracellular signal-regulated kinase
- differentiation
- proliferation
- E-cadherin
- beta-catenin