Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Marschitz, I; Tinhofer, I; Hittmair, A; Egle, A; Kos, M; Greil, R.
Analysis of Bcl-2 protein expression in chronic lymphocytic leukemia. A comparison of three semiquantitation techniques.
Am J Clin Pathol. 2000; 113(2):219-229
Doi: 10.1309/491W-L1TN-UFQX-T61B
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- Führende Autor*innen der Med Uni Graz
- Marschitz Ingrid Christine
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- Abstract:
- A number of studies revealed that high expression of the proto-oncogene bcl-2 correlated with poor prognosis or resistance to chemotherapy in some tumors but predicted a favorable clinical course in other neoplasias. In these studies, however, different immunologic techniques for Bcl-2 detection were used, raising the question of whether the methods applied were comparable. Using chronic lymphocytic leukemia (CLL) cells, the aims of our study were as follows: (1) to determine the reproducibility of Bcl-2 semiquantitation by immunocytochemistry, flow cytometry, or immunoblotting; (2) to study the agreement between results obtained by these methods; and (3) to examine the association between Bcl-2 expression in tumor cells of 99 patients with CLL and clinical parameters. We found that determination of Bcl-2 expression by immunocytochemistry was reproducible and the results were comparable with those of flow cytometry and immunoblotting. In the patient collective examined, Bcl-2 expression did not reflect the extent of tumor mass, but higher levels were found more often in patients with progressive disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Comparative Study -
- Flow Cytometry -
- Humans -
- Image Processing, Computer-Assisted -
- Immunoblotting -
- Immunohistochemistry -
- Leukemia, Lymphocytic, Chronic - metabolism
- Lymphocyte Count - metabolism
- Prognosis - metabolism
- Proto-Oncogene Proteins c-bcl-2 - analysis
- Quality Control - analysis
- Reproducibility of Results - analysis
- Research Support, Non-U.S. Gov't - analysis
- Staining and Labeling - analysis
- Find related publications in this database (Keywords)
- bcl-2 protein
- staining intensity
- semiquantitation methods
- reproducibility
- cutoff level for positivity
- chronic lymphocytic
- leukemia
- CLL
- clinical relevance