Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Steyrer, E; Barber, DL; Schneider, WJ.
Evolution of lipoprotein receptors. The chicken oocyte receptor for very low density lipoprotein and vitellogenin binds the mammalian ligand apolipoprotein E.
J Biol Chem. 1990; 265(32): 19575-19581.
Doi: 10.1016/S0021-9258(17)45410-X
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- Führende Autor*innen der Med Uni Graz
- Steyrer Ernst
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- Abstract:
- The laying hen expresses two different lipoprotein transport receptors in cell-specific fashion. On the one hand, a 95-kDa oocyte membrane protein mediates the uptake of the major yolk precursors, very low density lipoprotein, and vitellogenin; on the other hand, somatic cells synthesize a 130-kDa receptor that is involved in the regulation of cellular cholesterol homeostasis (Hayashi, K., Nimpf, J., and Schneider, W. J. (1989) J. Biol. Chem. 264, 3131-3139). Here we show that the oocyte-specific receptor binds, in addition to the yolk precursor proteins, an apolipoprotein of mammalian origin, apolipoprotein E. Ligand blotting, a solid-phase binding assay, and antireceptor antibodies were employed to demonstrate that binding of vitellogenin, very low density lipoprotein (via apolipoprotein B), and apolipoprotein E occurs to closely related, if not identical, sites on the 95-kDa oocyte receptor. The binding properties of lipovitellin, which harbors the receptor recognition site of vitellogenin, are analogous to those of apolipoprotein E: both require association with lipid for expression of functional receptor binding. The ligand specificity of the avian oocyte lipoprotein receptor supports the hypothesis that vitellogenin, which has evolved in oviparous species, represents a counterpart to mammalian apolipoprotein E.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apolipoproteins E - metabolism
- Centrifugation, Density Gradient - metabolism
- Chickens - metabolism
- Collodion - metabolism
- Electrophoresis, Polyacrylamide Gel - metabolism
- Female - metabolism
- Immunoblotting - metabolism
- Lipoproteins, VLDL - metabolism
- Male - metabolism
- Oocytes - metabolism
- Rabbits - metabolism
- Rats - metabolism
- Rats, Inbred Strains - metabolism
- Receptors, Cell Surface - metabolism
- Receptors, Lipoprotein - metabolism
- Vitellogenins - metabolism