Medizinische Universität Graz - Research portal
Selected Publication:
Esterbauer, H; Striegl, G; Puhl, H; Rotheneder, M.
Continuous monitoring of in vitro oxidation of human low density lipoprotein.
Free Radic Res Commun. 1989; 6(1): 67-75.
Doi: 10.3109/10715768909073429
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- Co-authors Med Uni Graz
- Dieber-Rotheneder Martina
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- Abstract:
- The kinetics of the oxidation of human low density lipoprotein (LDL) can be measured continuously by monitoring the change of the 234 nm diene absorption. The time-course shows three consecutive phases, a lag-phase during which the diene absorption increases only weakly, a propagation phase with a rapid increase of the diene absorption and finally a decomposition phase. The increase of the dienes is highly correlated with the increase of MDA or lipid hydroperoxides. The duration of the lag-phase is determined by the endogenous antioxidants contained in LDL (vitamin E, carotenoids, retinylstearate). Water-soluble antioxidants (ascorbic acid, urate) added in micromolar concentrations prolong the lag-phase in a concentration-dependent manner. The determination of the lag-phase is a convenient and objective procedure for determining the susceptibility of LDL from different donors towards oxidation as well as effects of pro- and antioxidants.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
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- Humans - blood
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- Monitoring, Physiologic - blood
- Oxidation-Reduction - blood
- Spectrophotometry, Ultraviolet - methods
- Vitamin E - blood