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Holzer, P; Jocic, M.
Cutaneous vasodilatation induced by nitric oxide-evoked stimulation of afferent nerves in the rat.
BRIT J PHARMACOL 1994 112: 1181-1187.
Doi: 10.1111/j.1476-5381.1994.tb13208.x
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- Leading authors Med Uni Graz
- Holzer Peter
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- Abstract:
- 1. The site of action at which nitric oxide (NO) may contribute to neurogenic vasodilatation in the hindpaw skin of urethane-anaesthetized rats was examined by the use of NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NO synthase. 2. Skin blood flow was measured by laser Doppler flowmetry, and neurogenic vasodilatation was evoked either by topical application of mustard oil (5%) or antidromic electrical stimulation of the saphenous nerve (antidromic vasodilatation). 3. L-NAME (60 mumol kg-1, i.v.) attenuated the hyperaemia evoked by mustard oil in an enantiomer-specific manner but failed to reduce antidromic vasodilatation and the vasodilatation due to i.v. injected calcitonin gene-related peptide (CGRP) and substance P (0.1-1 nmol kg-1 each), two proposed mediators of neurogenic vasodilatation. 4. Pretreatment of rats with capsaicin (125 mg kg-1, s.c. 2 weeks beforehand), to defunctionalize afferent neurones, reduced the hyperaemic response to mustard oil and prevented L-NAME from further decreasing the vasodilatation evoked by mustard oil. 5. Intraplantar infusion of sodium nitroprusside (SNP, 0.15 nmol in 1 min), a donor of NO, induced hyperaemia which was significantly diminished by the CGRP antagonist CGRP8-37 (50 nmol kg-1, i.v.) and by capsaicin pretreatment. The ability of CGRP8-37 to inhibit the vasodilator response to SNP was lost in capsaicin-pretreated rats. 6. Taken together, these data indicate that NO does not play a vasorelaxant messenger role in neurogenic vasodilatation but can contribute to activation of, and/or transmitter release from, afferent nerve fibres in response to irritant chemicals.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Arginine - analogs and derivatives
- Calcitonin Gene-Related Peptide - pharmacology
- Capsaicin - pharmacology
- Female - pharmacology
- Indoles - pharmacology
- NG-Nitroarginine Methyl Ester - pharmacology
- Nitric Oxide - physiology
- Nitroprusside - pharmacology
- Peptide Fragments - pharmacology
- Rats - pharmacology
- Rats, Sprague-Dawley - pharmacology
- Regional Blood Flow - drug effects
- Research Support, Non-U.S. Gov't - drug effects
- Skin - blood supply
- Substance P - pharmacology
- Vasodilation - drug effects
- Find related publications in this database (Keywords)
- Nitric Oxide
- N-G-Nitro-L-Arginine Methyl Ester (L-Name)
- Capsaicin
- Mustard Oil
- Sodium Nitroprusside
- Calcitonin Gene-Related Peptide (CGRP)
- Substance P
- Afferent Nerve Stimulation
- Skin Blood Flow
- Antidromic Vasodilatation