Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Desoye, G; Schauenstein, E.
Model experiments for quantitative cytophotometry using protein films (author's transl)
Microsc Acta. 1981; 84(1):9-16
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Desoye Gernot
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- Abstract:
- The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Carcinoma, Ehrlich Tumor - pathology
- Histocytochemistry - methods
- Proteins -
- Reference Standards -
- Spectrophotometry -
- Staining and Labeling -